• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Cold-Responsive Regions of Paradigm Cold-Shock and Non-Cold-Shock mRNAs Responsible for Cold Shock Translational Bias
【2h】

Cold-Responsive Regions of Paradigm Cold-Shock and Non-Cold-Shock mRNAs Responsible for Cold Shock Translational Bias

机译：范式冷休克和非冷休克mRNA的冷响应区域负责冷休克的翻译偏见。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

In Escherichia coli, the mRNA transcribed from the main cold-shock gene cspA is a thermosensor, which at low temperature adopts a conformation particularly suitable for translation in the cold. Unlike cspA, its paralogue cspD is expressed only at 37 °C, is toxic so cannot be hyper-expressed in E. coli and is poorly translated in vitro, especially at low temperature. In this work, chimeric mRNAs consisting of different segments of cspA and cspD were constructed to determine if parts of cspA could confer cold-responsive properties to cspD to improve its expression. The activities of these chimeric mRNAs in translation and in partial steps of translation initiation such as formation of 30S initiation complexes and 50S subunits docking to 30S complexes to yield 70S initiation complexes were analyzed. We show that the 5′ untranslated region (5′UTR) of cspA mRNA is sufficient to improve the translation of cspD mRNA at 37 °C whereas both the 5′UTR and the region immediately downstream the cspA mRNA initiation triplet are essential for translation at low temperature. Furthermore, the translational apparatus of cold-stressed cells contains trans-active elements targeting both 5′UTR and downstream regions of cspA mRNA, thereby improving translation of specific chimeric constructs at both 15 and 37 °C.
机译：在大肠杆菌中，从冷休克主要基因cspA转录的mRNA是一种热传感器，在低温下采用的构象特别适合在冷条件下翻译。与cspA不同，它的旁系同源cspD仅在37°C时表达，有毒，因此不能在大肠杆菌中过表达，并且在体外翻译不佳，尤其是在低温下。在这项工作中，构建了由cspA和cspD的不同片段组成的嵌合mRNA，以确定cspA的某些部分是否可以赋予cspD冷响应特性以改善其表达。分析了这些嵌合mRNA在翻译和翻译起始的部分步骤中的活性，例如形成30S起始复合物和与30S复合物对接以产生70S起始复合物的50S亚基。我们显示cspA mRNA的5'非翻译区（5'UTR）足以改善cspD mRNA在37°C时的翻译，而5'UTR和cspA mRNA起始三联体紧接下游的区域对于在低温。此外，冷应激细胞的翻译装置包含靶向5'UTR和cspA mRNA下游区域的反式活性元件，从而改善了15和37°C下特定嵌合构建体的翻译。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号