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首页> 美国卫生研究院文献>International Journal of Microbiology >The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea
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The Sulfate-Rich and Extreme Saline Sediment of the Ephemeral Tirez Lagoon: A Biotope for Acetoclastic Sulfate-Reducing Bacteria and Hydrogenotrophic Methanogenic Archaea

机译：短暂的蒂尔兹泻湖的硫酸盐丰富和极富盐分的沉积物：乙酰破伤菌减少细菌和氢营养型产甲烷古生菌的生物群落。

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摘要

Our goal was to examine the composition of methanogenic archaea (MA) and sulfate-reducing (SRP) and sulfur-oxidizing (SOP) prokaryotes in the extreme athalassohaline and particularly sulfate-rich sediment of Tirez Lagoon (Spain). Thus, adenosine-5′-phosphosulfate (APS) reductase α (aprA) and methyl coenzyme M reductase α (mcrA) gene markers were amplified given that both enzymes are specific for SRP, SOP, and MA, respectively. Anaerobic populations sampled at different depths in flooded and dry seasons from the anoxic sediment were compared qualitatively via denaturing gradient gel electrophoresis (DGGE) fingerprint analysis. Phylogenetic analyses allowed the detection of SRP belonging to Desulfobacteraceae, Desulfohalobiaceae, and Peptococcaceae in ∂-proteobacteria and Firmicutes and SOP belonging to Chromatiales/Thiotrichales clade and Ectothiorhodospiraceae in γ-proteobacteria as well as MA belonging to methylotrophic species in Methanosarcinaceae and one hydrogenotrophic species in Methanomicrobiaceae. We also estimated amino acid composition, GC content, and preferential codon usage for the AprA and McrA sequences from halophiles, nonhalophiles, and Tirez phylotypes. Even though our results cannot be currently conclusive regarding the halotolerant strategies carried out by Tirez phylotypes, we discuss the possibility of a plausible “salt-in” signal in SRP and SOP as well as of a speculative complementary haloadaptation between salt-in and salt-out strategies in MA.
机译：我们的目标是检查极度卤代卤代甲烷中特有的产甲烷古细菌（MA）和硫酸盐还原（SRP）和硫氧化（SOP）原核生物的组成，尤其是蒂尔泻湖（西班牙）的富含硫酸盐的沉积物。因此，假设两种酶分别对SRP，SOP和MA特异，则扩增了5'-磷酸腺苷（APS）还原酶α（aprA）和甲基辅酶M还原酶α（mcrA）基因标记。通过变性梯度凝胶电泳（DGGE）指纹分析定性地比较了缺水沉积物在缺水和干旱季节不同深度和深度的厌氧菌种群。通过系统发育分析，可以检测出te-变形杆菌和沙门氏菌中脱硫杆菌科，脱硫盐菌科和肽球菌科的SRP，以及在γ-变形杆菌属的甲基杆菌属中的嗜铬菌种和嗜藻菌属的嗜麦芽孢杆菌属中的嗜铬菌属，硫代拟杆菌科和嗜硫菌科甲烷微菌科。我们还估计了来自嗜盐菌，非嗜盐菌和Tirez系统型的AprA和McrA序列的氨基酸组成，GC含量和优先密码子使用。即使我们的结果目前尚无法就Tirez系统型所执行的卤代烷策略做出结论，但我们仍讨论了SRP和SOP中可能出现“盐入”信号的可能性，以及盐入和盐分之间的推测性互补重载的可能性。在MA的策略。

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