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首页> 美国卫生研究院文献>International Journal of Medical Sciences >FasL Expression on Human Nucleus Pulposus Cells Contributes to the Immune Privilege of Intervertebral Disc by Interacting with Immunocytes
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FasL Expression on Human Nucleus Pulposus Cells Contributes to the Immune Privilege of Intervertebral Disc by Interacting with Immunocytes

机译：通过与免疫细胞相互作用人髓核细胞上的FasL表达有助于椎间盘的免疫特权。

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摘要

The mechanisms of immune privilege in human nucleus pulposus (NP) remain unclear. Accumulating evidence indicates that Fas ligand (FasL) might play an important role in the immune privilege of the disc. We aimed for addressing the role of FasL expression in human intervertebral disc degeneration (IDD) and immune privilege in terms of the interaction between NP cells and immunocytes via the FasL-Fas machinery. We collected NP specimens from 20 patients with IDD as degenerative group and 8 normal cadaveric donors as control. FasL expression was detected by qRT-PCR, western blotting and flow cytometry (FCM). We also collected macrophages and CD8+ T cells from the peripheral blood of patients with IDD for co-cultures with NP cells. And macrophages and CD8+ T cells were harvested for apoptosis analysis by FCM after 2 days of co-cultures. We found that FasL expression in mRNA, protein and cellular resolutions demonstrated a significant decrease in degenerative group compared with normal control (p<0.05). FCM analysis found that human NP cells with increased FasL expression resulted in significantly increased apoptosis ratio of macrophages and CD8+ T cells. Our study demonstrated that FasL expression tends to decrease in degenerated discs and FasL plays an important role in human disc immune privilege, which might provide a novel target for the treatment strategies for IDD.
机译：人类髓核（NP）免疫特权的机制仍不清楚。越来越多的证据表明Fas配体（FasL）可能在光盘的免疫特权中起重要作用。我们旨在解决FasL表达在人椎间盘退变（IDD）和免疫特权方面的作用，即通过FasL-Fas机制在NP细胞和免疫细胞之间的相互作用。我们收集了20例IDD患者的NP标本作为变性组，将8例正常尸体供体作为对照。通过qRT-PCR，蛋白质印迹和流式细胞术（FCM）检测FasL表达。我们还从IDD患者的外周血中收集巨噬细胞和CD8 + T细胞与NP细胞共培养。共培养2天后，收集巨噬细胞和CD8 + T细胞用于FCM的凋亡分析。我们发现，与正常对照组相比，变性组的FasL在mRNA，蛋白质和细胞分辨率中的表达明显降低（p <0.05）。 FCM分析发现FasL表达增加的人NP细胞导致巨噬细胞和CD8 + T细胞的凋亡率显着增加。我们的研究表明，FasL表达在退化的椎间盘中趋于减少，并且FasL在人椎间盘免疫特权中起重要作用，这可能为IDD的治疗策略提供了新的靶点。

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