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首页> 美国卫生研究院文献>International Journal of Environmental Research and Public Health >Multidrug Resistance and Plasmid Patterns of Escherichia coli O157 and Other E. coli Isolated from Diarrhoeal Stools and Surface Waters from Some Selected Sources in Zaria Nigeria
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Multidrug Resistance and Plasmid Patterns of Escherichia coli O157 and Other E. coli Isolated from Diarrhoeal Stools and Surface Waters from Some Selected Sources in Zaria Nigeria

机译：从尼日利亚扎里亚一些选定来源的腹泻粪便和地表水中分离的大肠杆菌O157和其他大肠杆菌的多药耐药性和质粒模式

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摘要

We have assessed the prevalence of Escherichia coli O157 in diarrhoeal patients and surface waters from some selected sources in Zaria (Nigeria), evaluating the antibiotic susceptibility and plasmid profiles of 184 E. coli isolates, obtained from 228 water samples and 112 diarrhoeal stool specimens (collected from children aged <15 years), using standard methods. The detection rate of E. coli O157 in surface waters was 2.2% and its prevalence in children with diarrhoea was 5.4%. The most active antibiotics were gentamicin, chloramphenicol and fluoroquinolones. Seventy-nine (42.9%) of 184 E. coli isolates were resistant to four or more antibiotics. Multidrug resistance (MDR) was higher amongst aquatic isolates than the clinical isolates. Out of 35 MDR isolates (20 of which were O157 strains), 22 (62.9%) harboured plasmids all of which were no less than 2.1 kb in size. Amongst the 20 E. coli O157 strains, only seven (35.0%) contained multiple plasmids. An aquatic O157 isolate containing two plasmids was resistant to seven drugs, including ampicillin, cefuroxime, ciprofloxacin, cotrimoxazole, nalidixic acid, nitrofurantoin and tetracycline. Loss of plasmid correlated with loss of resistance to antibiotics in cured (mutant) strains selected in tetracycline (50 μg/mL)-nutrient agar plates. Our findings revealed that plasmids were prevalent in both the aquatic and clinical isolates, and suggest that the observed MDR is plasmid-mediated. The occurrence of plasmid-mediated multidrug resistant E. coli O157 in surface waters used as sources for drinking, recreation and fresh produce irrigation heightens public health concern.
机译：我们评估了腹泻患者和来自Zaria（尼日利亚）某些选定来源的地表水中大肠杆菌O157的患病率，评估了从228份水样和112份腹泻粪便样本中获得的184株大肠杆菌的抗生素敏感性和质粒图谱（使用标准方法从15岁以下儿童处收集）。大肠杆菌O157在地表水中的检出率为2.2％，其在腹泻儿童中的患病率为5.4％。活性最高的抗生素是庆大霉素，氯霉素和氟喹诺酮。 184株大肠杆菌中有79株（42.9％）对四种或更多种抗生素具有抗药性。水生分离株中的多药耐药性（MDR）高于临床分离株。在35个MDR分离株中（其中20个是O157菌株），有22个（62.9％）带有质粒，所有质粒的大小均不小于2.1 kb。在20个大肠杆菌O157菌株中，只有七个（35.0％）包含多个质粒。含有两个质粒的水产O157分离株对七种药物有抗性，包括氨苄西林，头孢呋辛，环丙沙星，cotrimoxazole，萘啶酸，呋喃妥因和四环素。在四环素（50μg/ mL）营养琼脂平板中选择的治愈（突变）菌株中，质粒的丧失与对抗生素的抗性丧失相关。我们的发现表明，质粒在水生和临床分离株中均很普遍，并且表明所观察到的MDR是质粒介导的。在用作饮用水，娱乐和新鲜农产品灌溉源的地表水中，质粒介导的多药耐药大肠杆菌O157的出现引起了公众健康的关注。

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