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Identification and quantification of glucosinolate and flavonol compounds in rocket salad (Eruca sativa Eruca vesicaria and Diplotaxis tenuifolia) by LC–MS: Highlighting the potential for improving nutritional value of rocket crops

机译:LC-MS鉴定和定量火箭沙拉中的芥子油苷和黄酮醇类化合物(Eruca sativaEruca vesicaria和Diplotaxis tenuifolia):突出了改善火箭作物营养价值的潜力

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摘要

Liquid chromatography mass spectrometry (LC–MS) was used to obtain glucosinolate and flavonol content for 35 rocket accessions and commercial varieties. 13 glucosinolates and 11 flavonol compounds were identified. Semi-quantitative methods were used to estimate concentrations of both groups of compounds. Minor glucosinolate composition was found to be different between accessions; concentrations varied significantly. Flavonols showed differentiation between genera, with Diplotaxis accumulating quercetin glucosides and Eruca accumulating kaempferol glucosides. Several compounds were detected in each genus that have only previously been reported in the other. We highlight how knowledge of phytochemical content and concentration can be used to breed new, nutritionally superior varieties. We also demonstrate the effects of controlled environment conditions on the accumulations of glucosinolates and flavonols and explore the reasons for differences with previous studies. We stress the importance of consistent experimental design between research groups to effectively compare and contrast results.
机译:液相色谱质谱法(LC-MS)用于获得35种火箭种和商业品种的芥子油苷和黄酮醇含量。鉴定出13种芥子油苷和11种黄酮醇化合物。使用半定量方法估算两组化合物的浓度。发现次要芥子油苷组成不同。浓度差异很大。黄酮醇显示属之间的分化,Diplotaxis累积槲皮素葡萄糖苷,而Eruca累积kaempferol葡萄糖苷。在每个属中检测到几种化合物,而以前仅在其他属中报告过。我们着重介绍如何利用植物化学成分和浓度的知识来培育营养上优越的新品种。我们还证明了受控环境条件对芥子油苷和黄酮醇积累的影响,并探讨了与先前研究差异的原因。我们强调研究组之间一致的实验设计对有效比较和对比结果的重要性。

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