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首页> 美国卫生研究院文献>Frontiers in Genetics >Aquaculture Breeding Enhancement: Maturation and Spawning in Sea Cucumbers Using a Recombinant Relaxin-Like Gonad-Stimulating Peptide
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Aquaculture Breeding Enhancement: Maturation and Spawning in Sea Cucumbers Using a Recombinant Relaxin-Like Gonad-Stimulating Peptide

机译：水产养殖育种增强：使用重组松弛素样性腺刺激肽在海参中成熟和产卵

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Wild sea cucumber resources have been rapidly exhausted and therefore there is an urgent need to develop approaches that will help restocking. Currently, there is a lack of information regarding the genes involved in sea cucumber reproductive processes. The neurohormone relaxin-like gonad-stimulating peptide (RGP) has been identified as the active gonad-stimulating peptide in sea stars (Asteroidea), which could also be present in other echinoderm groups. In this study, a sea cucumber RGP was identified and confirmed by phylogenetic analysis. A recombinant Holothuria scabra RGP was produced in the yeast Pichia pastoris and confirmed by mass spectrometry. To assess bioactivity, four levels of purification were tested in an in vitro germinal vesicle breakdown (GVBD) bioassay. The most pure form induced 98.56 ± 1.19% GVBD in H. scabra and 89.57 ± 1.19% GVBD in Holothuria leucospilota. Cruder levels of purification still resulted in some GVBD. Upon single injection into female H. scabra, the recombinant RGP induced head waving behavior followed by spawning within 90–170 min. Spawned oocytes were fertilized successfully, larvae settled and developed into juveniles. Our results provide a key finding for the development of a break-through new artificial breeding approach in sea cucumber aquaculture.
机译：野生海参资源已迅速枯竭，因此迫切需要开发有助于补给的方法。当前，缺乏有关海参生殖过程中涉及的基因的信息。神经激素松弛素样性腺刺激肽（RGP）已被确定为海星（Asteroidea）中的活性性腺刺激肽，也可能存在于其他棘皮动物组中。在这项研究中，通过系统发育分析鉴定并确认了海参RGP。在酵母巴斯德毕赤酵母中产生了重组胡萝苏假单胞菌RGP，并通过质谱法证实。为了评估生物活性，在体外生发小泡分解（GVBD）生物测定中测试了四个纯化水平。最纯净的形式在H豆中诱导出98.56±1.19％的GVBD，在白带整枝者中诱导了89.57±1.19％的GVBD。粗级纯化仍然产生一些GVBD。当单次注射到雌性H. scabra中时，重组RGP引起头挥动行为，然后在90–170分钟内产卵。产生的卵母细胞成功受精，幼虫定居并发育为幼体。我们的结果为海参水产养殖中突破性的新型人工育种方法的开发提供了关键发现。

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