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Microbial methane formation in deep aquifers of a coal-bearing sedimentary basin, Germany

机译:德国含煤沉积盆地深层含水层中的微生物甲烷形成

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摘要

Coal-bearing sediments are major reservoirs of organic matter potentially available for methanogenic subsurface microbial communities. In this study the specific microbial community inside lignite-bearing sedimentary basin in Germany and its contribution to methanogenic hydrocarbon degradation processes was investigated. The stable isotope signature of methane measured in groundwater and coal-rich sediment samples indicated methanogenic activity. Analysis of 16S rRNA gene sequences showed the presence of methanogenic Archaea, predominantly belonging to the orders Methanosarcinales and Methanomicrobiales, capable of acetoclastic or hydrogenotrophic methanogenesis. Furthermore, we identified fermenting, sulfate-, nitrate-, and metal-reducing, or acetogenic Bacteria clustering within the phyla Proteobacteria, complemented by members of the classes Actinobacteria, and Clostridia. The indigenous microbial communities found in the groundwater as well as in the coal-rich sediments are able to degrade coal-derived organic components and to produce methane as the final product. Lignite-bearing sediments may be an important nutrient and energy source influencing larger compartments via groundwater transport.
机译:含煤沉积物是潜在用于产甲烷的地下微生物群落的主要有机物储集层。在这项研究中,研究了德国含褐煤沉积盆地中特定的微生物群落及其对甲烷生成烃降解过程的贡献。在地下水和富含煤的沉积物样品中测得的甲烷的稳定同位素特征表明产甲烷活性。对16S rRNA基因序列的分析表明,存在产甲烷的古细菌,主要属​​于产甲烷菌和甲烷微生物的顺序,能够进行乙酰碎裂或氢营养的产甲烷作用。此外,我们确定了门菌属细菌中的发酵,硫酸盐,硝酸盐和金属还原或产乙酸细菌聚集,并辅以放线菌和梭状芽胞杆菌。在地下水以及富含煤炭的沉积物中发现的土著微生物群落能够降解煤炭衍生的有机成分,并产生甲烷作为最终产物。含褐煤的沉积物可能是重要的养分和能源,通过地下水运输影响较大的隔室。

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