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Polar front associated variation in prokaryotic community structure in Arctic shelf seafloor

机译:北极大陆架海底极地锋相关原核生物群落结构的变化

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摘要

Spatial variations in composition of marine microbial communities and its causes have largely been disclosed in studies comprising rather large environmental and spatial differences. In the present study, we explored if a moderate but temporally permanent climatic division within a contiguous arctic shelf seafloor was traceable in the diversity patterns of its bacterial and archaeal communities. Soft bottom sediment samples were collected at 10 geographical locations, spanning spatial distances of up to 640 km, transecting the oceanic polar front in the Barents Sea. The northern sampling sites were generally colder, less saline, shallower, and showed higher concentrations of freshly sedimented phytopigments compared to the southern study locations. Sampling sites depicted low variation in relative abundances of taxa at class level, with persistent numerical dominance by lineages of Gamma- and Deltaproteobacteria (57–66% of bacterial sequence reads). The Archaea, which constituted 0.7–1.8% of 16S rRNA gene copy numbers in the sediment, were overwhelmingly (85.8%) affiliated with the Thaumarchaeota. Beta-diversity analyses showed the environmental variations throughout the sampling range to have a stronger impact on the structuring of both the bacterial and archaeal communities than spatial effects. While bacterial communities were significantly influenced by the combined effect of several weakly selective environmental differences, including temperature, archaeal communities appeared to be more uniquely structured by the level of freshly sedimented phytopigments.
机译:海洋微生物群落组成的空间变化及其成因已在包括相当大的环境和空间差异的研究中被披露。在本研究中,我们探讨了在连续的北极陆架海底内是否存在适度但暂时的气候分区,可以追溯到其细菌和古细菌群落的多样性。在巴伦支海的海洋极地横断面,在10个地理位置收集了软底沉积物样本,这些地理距离跨越了640 km。与南部研究地点相比,北部取样地点通常较冷,盐分较少,较浅,并显示出较高浓度的新鲜沉积的植物色素。取样位点显示,在分类水平上,分类单元的相对丰度变化不大,γ-和Deltaproteobacteria谱系的谱系优势持续存在(占细菌序列读数的57-66%)。古细菌占沉积物中16S rRNA基因拷贝数的0.7–1.8%,绝大多数(85.8%)与Thaumarchaeota相关。 Beta多样性分析表明,整个采样范围内的环境变化对细菌和古细菌群落的结构产生的影响要大于空间影响。虽然细菌群落受到包括温度在内的几种弱选择性环境差异的综合影响,但古细菌群落似乎由新鲜沉积的植物色素的水平更为独特。

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