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首页> 美国卫生研究院文献>Immunology >Functional and Vβ repertoire characterization of human CD8+ T-cell subsets with natural killer cell markers, CD56+ CD57− T cells, CD56+ CD57+ T cells and CD56− CD57+ T cells
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Functional and Vβ repertoire characterization of human CD8+ T-cell subsets with natural killer cell markers, CD56+ CD57− T cells, CD56+ CD57+ T cells and CD56− CD57+ T cells

机译：具有自然杀伤细胞标记，CD56 + CD57- T细胞，CD56 + CD57 + T细胞和CD56- CD57 + T细胞的人CD8 + T细胞亚群的功能和Vβ谱系表征

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We investigated the individual CD8+ populations with natural killer (NK) cell markers (NK-type T cell); CD56 single positive (CD56)-T cells, CD56/CD57 double positive (DP)-T cells and CD57 single positive (CD57)-T cells in the peripheral blood. All NK-type T-cell populations expressed CD122 and intermediate levels of T-cell receptor (TCR; regular CD8+ T cells are CD122− and express high levels of TCR). The number of both DP-T cells and CD57-T cells, but not CD56-T cells, gradually increased with age. All NK-type T-cell populations produced larger amounts of interferon-γ than did regular CD8+ T cells after stimulation with interleukin (IL)-2, IL-12 and IL-15. However, CD56-T cells and CD57-T cells but not DP-T cells showed a potent antitumour cytotoxity to NK-sensitive K562 cells, whereas only CD56-T cells showed a potent cytotoxity to NK-resistant Raji cells. Furthermore, although NK-type T cells produced large amounts of soluble Fas-ligands, their cytotoxic activities appeared to be mediated by the perforin/granzyme pathway. The oligoclonal or pauciclonal expansions of certain VβT cells were found in each NK-type T-cell population. The non-variant CDR3 region(s) for the TCRβ chain(s) showed CD57-T cells and CD56-T cells to be derived from distinct origins, while the DP-T cell population consisted of a mixture of the clones seen in both CD56-T cells and CD57-T cells. Our results suggest that CD57-T cells and CD56-T cells are functionally and ontogenically different populations while DP-T cells appear to originate from both CD56-T cells and CD57-T cells.
机译：我们调查了具有自然杀伤（NK）细胞标记（NK型T细胞）的单个CD8 + 种群；外周血中的CD56单阳性（CD56）-T细胞，CD56 / CD57双阳性（DP）-T细胞和CD57单阳性（CD57）-T细胞。所有NK型T细胞群体均表达CD122和中等水平的T细胞受体（TCR；常规CD8 + T细胞为CD122 -，并表达高水平的TCR）。 。随着年龄的增长，DP-T细胞和CD57-T细胞（而不是CD56-T细胞）的数量逐渐增加。白介素（IL）-2，IL-12和IL-15刺激后，所有NK型T细胞群体产生的干扰素-γ数量均比常规CD8 + T细胞更大。但是，CD56-T细胞和CD57-T细胞而非DP-T细胞对NK敏感的K562细胞表现出有效的抗肿瘤细胞毒性，而只有CD56-T细胞对NK耐药的Raji细胞表现出有效的细胞毒性。此外，尽管NK型T细胞产生大量的可溶性Fas配体，但它们的细胞毒性活性似乎是由穿孔素/粒酶途径介导的。在每个NK型T细胞群体中都发现了某些VβT细胞的寡克隆或丘脑扩张。 TCRβ链的非变异CDR3区显示CD57-T细胞和CD56-T细胞来自不同的起源，而DP-T细胞群体由两个克隆的混合物组成CD56-T细胞和CD57-T细胞。我们的结果表明，CD57-T细胞和CD56-T细胞在功能和基因组上是不同的，而DP-T细胞似乎起源于CD56-T细胞和CD57-T细胞。

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