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首页> 美国卫生研究院文献>International Journal of Molecular Sciences >Overcoming the Recalcitrance for the Conversion of Kenaf Pulp to Glucose via Microwave-Assisted Pre-Treatment Processes
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Overcoming the Recalcitrance for the Conversion of Kenaf Pulp to Glucose via Microwave-Assisted Pre-Treatment Processes

机译：克服通过微波辅助预处理工艺将红麻纸浆转化为葡萄糖的顽固性

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This study evaluates the pre-treatment of cellulose from kenaf plant to yield sugar precursors for the production of ethanol or butanol for use as biofuel additives. In order to convert the crystalline cellulosic form to the amorphous form that can undergo enzymatic hydrolysis of the glycosidic bond to yield sugars, kenaf pulp samples were subjected to two different pre-treatment processes. In the acid pre-treatment, the pulp samples were treated with 37.5% hydrochloric acid in the presence of FeCl3 at 50 °C or 90 °C whereas in the alkaline method, the pulp samples were treated with 25% sodium hydroxide at room temperature and with 2% or 5% sodium hydroxide at 50 °C. Microwave-assisted NaOH-treatment of the cellulose was also investigated and demonstrated to be capable of producing high glucose yield without adverse environmental impact by circumventing the use of large amounts of concentrated acids i.e., 83–85% phosphoric acid employed in most digestion processes. The treated samples were digested with the cellulase enzyme from Trichoderma reesei. The amount of glucose produced was quantified using the Quantichrom™ glucose bioassay for assessing the efficiency of glucose production for each of the treatment processes. The microwave-assisted alkaline pre-treatment processes conducted at 50 °C were found to be the most effective in the conversion of the crystalline cellulose to the amorphous form based on the significantly higher yields of sugar produced by enzymatic hydrolysis compared to the untreated sample.
机译：这项研究评估了洋麻植物中纤维素的预处理，以制得糖前体，以生产乙醇或丁醇用作生物燃料添加剂。为了将结晶的纤维素形式转化为可以经历糖苷键的酶促水解以产生糖的无定形形式，对洋麻纸浆样品进行了两种不同的预处理过程。在酸预处理中，在FeCl3存在下于50°C或90°C下，用37.5％的盐酸处理纸浆样品，而在碱性方法中，在室温下用25％的氢氧化钠处理纸浆样品。在50°C下用2％或5％的氢氧化钠。还通过对纤维素进行微波辅助NaOH处理进行了研究，结果表明，通过避免在大多数消化过程中使用大量的浓酸，即83-85％的磷酸，可以在不产生不利环境影响的情况下产生高葡萄糖产量。用来自里氏木霉的纤维素酶消化处理过的样品。使用Quantichrom ™葡萄糖生物测定法对产生的葡萄糖量进行定量，以评估每个处理过程的葡萄糖产生效率。由于与未处理的样品相比，通过酶促水解产生的糖的产率明显更高，因此发现在50°C进行的微波辅助碱预处理过程最有效地将结晶纤维素转化为无定形形式。

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