• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Journal of Anatomy >HSP110, caspase-3 and -9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all-trans retinoic acid or irradiation during early mouse eye development
【2h】

HSP110, caspase-3 and -9 expression in physiological apoptosis and apoptosis induced by in vivo embryonic exposition to all-trans retinoic acid or irradiation during early mouse eye development

机译：HSP110，caspase-3和-9在小鼠胚胎发育早期的体内胚胎暴露于全反式维甲酸或辐射诱导的生理凋亡和凋亡中的表达

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Apoptosis is an essential physiological process in embryonic development. In the developing eye of vertebrates, three periods of developmental apoptosis can be distinguished: early, intermediate and later. Within the apoptosis pathway, caspases play a crucial role. It has also been shown that HSP110 may have a potential role in apoptosis. The aim of this research was to study the expression of HSP110, caspase-3 and -9 in physiological, retinoic- or irradiation-induced apoptosis during early eye development. Seven pregnant C57Bl/6J mice received 80 mg kg−1 of all-trans retinoic acid mixed with sesame oil. Seven pregnant NMRI mice received 2 Gy irradiation at the same gestational day. Control mice of both strains (seven mice of each) were not submitted to any treatment. Embryos were harvested at 3, 6, 12 and 24 h after exposition, fixed, dehydrated and embedded. Coronal sections (5 µm) were made. Slide staining occurred alternatively using anti-caspase-3, anti-caspase-9 and anti-HSP110 immunohistochemistry. HSP110 and caspase-3 expression presented similar topographic and chronological patterns, whereas expression of HSP110 was more precocious in retinoic acid-treated embryos. After retinoic exposure, caspase-3- and HSP110-positive cells were increased in the region of the optic vesicle. By contrast, after irradiation, caspase-3- and HSP110-positive cells were noticeably increased in the optic vesicle, peri-optical mesoderm but less in lens placode. HSP110 was expressed before caspase-3. By contrast, caspase-9 was expressed by a very small number of cells in the optic vesicle either under physiological or under teratogenic conditions. Thus, it seems that activation of caspase-9 is dispensable in early eye developmental apoptosis.
机译：细胞凋亡是胚胎发育中必不可少的生理过程。在脊椎动物的发育眼中，可以区分发育凋亡的三个阶段：早期，中期和后期。在凋亡途径中，胱天蛋白酶起着至关重要的作用。还显示出HSP110可能在细胞凋亡中具有潜在作用。这项研究的目的是研究HSP110，caspase-3和-9在早期眼睛发育过程中在生理，视黄酸或辐射诱导的细胞凋亡中的表达。七只怀孕的C57Bl / 6J小鼠接受了80 mg kg -1 全反式维甲酸与芝麻油的混合。七只怀孕的NMRI小鼠在同一孕周接受了2 Gy照射。两种品系的对照小鼠（每只小鼠七只）均未接受任何治疗。暴露后3、6、12和24小时收获胚胎，将其固定，脱水并包埋。制作了冠状切片（5 µm）。可以使用抗caspase-3，抗caspase-9和抗HSP110免疫组织化学方法进行切片染色。 HSP110和caspase-3的表达表现出相似的地形和时间顺序，而HSP110的表达在视黄酸处理的胚胎中更早熟。维甲酸暴露后，视神经囊泡区域caspase-3-和HSP110阳性细胞增加。相比之下，辐照后，半透明囊泡，中膜周围中胚层中的caspase-3-阳性细胞和HSP110阳性细胞明显增加，而在晶状体基底膜中则较少。 HSP110在caspase-3之前表达。相比之下，在生理或致畸条件下，视神经囊泡中的少量细胞表达caspase-9。因此，似乎caspase-9的激活在早期眼睛发育凋亡中是必不可少的。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号