Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri.

机译：甲烷甲烷八叠球菌的裂解物对乙酸的产甲烷裂解。

代理获取

本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。

页面导航

摘要
著录项
相似文献
相关主题

摘要

Cell lysates of acetate-grown Methanosarcina barkeri 227 were found to cleave acetate to CH4 and CO2. The aceticlastic reaction was identified by using radioactive methyl-labeled acetate. Cell lysates decarboxylated acetate in a nitrogen atmosphere, conserving the methyl group in methane. The rate of methanogenesis from acetate in the cell lysates was comparable to that observed with whole cells. Aceticlastic activity was found in the particulate fraction seperate from methylcoenzyme M methylreductase activity, which occurs in the soluble fraction. Pronase treatment eliminated methylcoenzyme M methylreductase activity in lysates and stimulated aceticlastic activity, indicating the aceticlastic activity was not derived from unbroken cells, which are unaffected by proteolytic treatment.

机译：发现乙酸盐生长的甲烷甲烷八叠球菌227的细胞裂解物将乙酸盐裂解为CH4和CO2。通过使用放射性甲基标记的乙酸酯鉴定了破弹反应。细胞在氮气氛中裂解脱羧乙酸盐，保留甲烷中的甲基。细胞裂解物中乙酸盐的甲烷生成速率与全细胞观察到的速率相当。在与可溶级分中发生的甲基辅酶M甲基还原酶活性分开的颗粒级分中发现了弹塑性活性。链霉蛋白酶处理消除了裂解物中的甲基辅酶M甲基还原酶活性，并刺激了抗破弹活性，这表明该抗破弹活性不是来自未破碎的细胞，而未受蛋白水解处理的影响。

著录项

期刊名称 Journal of Bacteriology
作者
L Baresi;
展开▼
作者单位

展开▼
年(卷),期 1937(160),1
年度 1937
页码 365–370
总页数 6
原文格式 PDF
正文语种
中图分类微生物学;
关键词

相似文献

外文文献
中文文献
专利

1. Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri. [J] . L Baresi Journal of bacteriology . 1984,第1期

机译：甲烷甲烷八叠球菌的裂解物对乙酸的产甲烷裂解。
2. Methyl-coenzyme M, an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri. [J] . S Shapiro, R S Wolfe Journal of bacteriology . 1980,第2期

机译：甲基辅酶M，是甲烷甲烷八叠球菌（甲烷化甲烷菌）甲烷分解C1化合物的中间体。
3. Substrate and accessory protein requirements and thermodynamics of acetyl-CoA synthesis and cleavage in Methanosarcina barkeri. [J] . Grahame DA, DeMoll E Biochemistry . 1995,第14期

机译：甲烷甲烷单孢菌中底物和辅助蛋白质的需求以及乙酰辅酶A合成和裂解的热力学。
4. Identification and Analysis of S-layer Proteins from Methanosarcina acetivorans and Methanosarcina mazei [C] . Deborah Leon-Rossell, Pinmanee Boontheung, Yanan Yang, ASMS Conference on Mass Spectrometry and Allied Topics . 2007

机译：来自甲蛋白酶醋酸甲蛋白腺体和甲蛋白菌Mazei的S层蛋白的鉴定及分析
5. Top-down proteomics: Separation and mass spectral characterization of complex cell lysates from Methanosarcina acetivorans and human cancer cells [D] . Ferguson, Jonathan T. 2008

机译：自上而下的蛋白质组学：分离自乙型甲烷单胞菌和人类癌细胞的复杂细胞裂解物的分离和质谱表征
6. Methyl-coenzyme M an intermediate in methanogenic dissimilation of C1 compounds by Methanosarcina barkeri. [O] . S Shapiro, R S Wolfe 1980

机译：甲基辅酶M是甲烷甲烷八叠球菌（甲烷化甲烷菌）甲烷分解C1化合物的中间体。
7. Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri [O] . L Baresi 1984

机译：甲基菌裂解物的乙酸甲酯切割裂解
8. Transmethylation reactions during methanogenesis from acetate in Methanosarcina barkeri. Annual technical progress report, August 1993--July 1994 [R] . Krzycki, J. A. 1994

机译：methanosarcina barkeri中乙酸甲烷生成过程中的甲基转移反应。年度技术进步报告，1993年8月至1994年7月

Methanogenic cleavage of acetate by lysates of Methanosarcina barkeri.

摘要

著录项

相似文献

相关主题

期刊订阅