The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing

机译：视网膜母细胞瘤基因产物的氨基末端区域结合了一种新的核基质蛋白该蛋白共定位于RNA加工中心

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The tumor suppressing capacity of the retinoblastoma protein (p110RB) is dependent on interactions made with cellular proteins through its carboxy-terminal domains. How the p110RB amino-terminal region contributes to this activity is unclear, though evidence now indicates it is important for both growth suppression and regulation of the full- length protein. We have used the yeast two-hybrid system to screen for cellular proteins which bind to the first 300 amino acids of p110RB. The only gene isolated from this screen encodes a novel 84-kD nuclear matrix protein that localizes to subnuclear regions associated with RNA processing. This protein, p84, requires a structurally defined domain in the amino terminus of p110RB for binding. Furthermore, both in vivo and in vitro experiments demonstrate that p84 binds preferentially to the functionally active, hypophosphorylated form of p110RB. Thus, the amino terminus of p110RB may function in part to facilitate the binding of growth promoting factors at subnuclear regions actively involved in RNA metabolism.

机译：视网膜母细胞瘤蛋白（p110RB）的肿瘤抑制能力取决于通过其羧基末端结构域与细胞蛋白发生的相互作用。尽管现在有证据表明p110RB氨基末端区域如何作用于这种活性，但尚不清楚，这对于生长抑制和全长蛋白质的调节都很重要。我们已经使用酵母双杂交系统来筛选与p110RB的前300个氨基酸结合的细胞蛋白。从该筛选中分离出的唯一基因编码一种新型的84 kD核基质蛋白，该蛋白定位于与RNA处理相关的亚核区域。该蛋白质p84需要在p110RB的氨基末端具有结构上定义的域才能结合。此外，体内和体外实验均证明p84优先与p110RB的功能活性，次磷酸化形式结合。因此，p110RB的氨基末端可以部分起作用以促进生长促进因子在积极参与RNA代谢的亚核区域的结合。

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期刊名称 The Journal of Biophysical and Biochemical Cytology
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年(卷),期 1994(127),3
年度 1994
页码 609–622
总页数 14
原文格式 PDF
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中图分类生物学;
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专利

1. The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing. [J] . T Durfee, M A Mancini, D Jones, Journal of cell biology . 1994,第3期

机译：视网膜母细胞瘤基因产物的氨基末端区域结合了一种新的核基质蛋白，该蛋白共定位于RNA处理中心。
2. SMAR1, a novel, alternatively spliced gene product, binds the Scaffold/Matrix-associated region at the T cell receptor beta locus. [J] . Chattopadhyay S, Kaul R, Charest A, Genomics . 2000,第1期

机译：SMAR1，一种新的或选择性剪接的基因产物，与T细胞受体β基因座处的支架/基质相关区域结合。
3. Molecular cloning of a cDNA encoding human SPH-binding factor, a conserved protein that binds to the enhancer-like region of the U6 small nuclear RNA gene promoter. [J] . Rincon JC, Engler SK, Hargrove BW, Nucleic Acids Research . 1998,第21期

机译：编码人SPH结合因子的cDNA的分子克隆，该蛋白是与U6小核RNA基因启动子的增强子样区域结合的保守蛋白。
4. RNA binding proteins (RBPs) regulate lncRNA nuclear retention [C] . Yile Huang, Yulong Qiao, Yu Zhao, IEEE International Conference on Bioinformatics and Biomedicine . 2020

机译：RNA结合蛋白（RBPS）调节LNCRNA核保留
5. High mobility group I /Y -type proteins in Nicotiana tabacum and their interactions with matrix attachment regions and the nuclear matrix [D] . Ingram, Jennifer Leigh 2000

机译：烟草中的高迁移率I / Y型蛋白质及其与基质附着区和核基质的相互作用
6. HIV-1 infection of non-dividing cells: evidence that the amino-terminal basic region of the viral matrix protein is important for Gag processing but not for post-entry nuclear import. [O] . R A Fouchier, B E Meyer, J H Simon, 1997

机译：HIV-1感染非分裂细胞：证据表明病毒基质蛋白的氨基末端碱性区域对于Gag加工很重要但对进入核后并不重要。
7. The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing [O] . 1994

机译：视网膜母细胞瘤基因产物的氨基末端区域结合了一种新的核基质蛋白，该蛋白共定位于RNA加工中心

The amino-terminal region of the retinoblastoma gene product binds a novel nuclear matrix protein that co-localizes to centers for RNA processing

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