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首页> 美国卫生研究院文献>The Journal of Biophysical and Biochemical Cytology >Characterization and Expression of the Laminin γ3 Chain: A Novel Non-Basement Membrane–associated Laminin Chain
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Characterization and Expression of the Laminin γ3 Chain: A Novel Non-Basement Membrane–associated Laminin Chain

机译：层粘连蛋白γ3链的表征和表达：一种新型的非基膜相关的层粘连蛋白链

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摘要

Laminins are heterotrimeric molecules composed of an α, a β, and a γ chain; they have broad functional roles in development and in stabilizing epithelial structures. Here, we identified a novel laminin, composed of known α and β chains but containing a novel γ chain, γ3. We have cloned gene encoding this chain, LAMC3, which maps to chromosome 9 at q31-34. Protein and cDNA analyses demonstrate that γ3 contains all the expected domains of a γ chain, including two consensus glycosylation sites and a putative nidogen-binding site. This suggests that γ3-containing laminins are likely to exist in a stable matrix.Studies of the tissue distribution of γ3 chain show that it is broadly expressed in: skin, heart, lung, and the reproductive tracts. In skin, γ3 protein is seen within the basement membrane of the dermal-epidermal junction at points of nerve penetration. The γ3 chain is also a prominent element of the apical surface of ciliated epithelial cells of: lung, oviduct, epididymis, ductus deferens, and seminiferous tubules. The distribution of γ3-containing laminins on the apical surfaces of a variety of epithelial tissues is novel and suggests that they are not found within ultrastructurally defined basement membranes. It seems likely that these apical laminins are important in the morphogenesis and structural stability of the ciliated processes of these cells.
机译：层粘连蛋白是由α，β和γ链组成的异三聚体分子；它们在发育和稳定上皮结构中具有广泛的功能作用。在这里，我们确定了一种新型层粘连蛋白，由已知的α和β链组成，但含有一条新颖的γ链γ3。我们已经克隆了编码该链的基因LAMC3，它映射到q31-34处的9号染色体。蛋白质和cDNA分析表明，γ3包含γ链的所有预期结构域，包括两个共有糖基化位点和一个假定的氮原结合位点。这表明含γ3的层粘连蛋白很可能存在于稳定的基质中.γ3链的组织分布研究表明，它在皮肤，心脏，肺和生殖道中广泛表达。在皮肤中，在神经穿透点的真皮-表皮连接处的基膜内可见到γ3蛋白。 γ3链也是纤毛上皮细胞顶表面的重要元素：肺，输卵管，附睾，输精管和生精小管。含γ3层粘连蛋白在各种上皮组织的顶表面上的分布是新颖的，这表明在超结构定义的基底膜中未发现它们。这些顶端层粘连蛋白似乎对这些细胞纤毛过程的形态发生和结构稳定性很重要。

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