• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>The Journal of Biophysical and Biochemical Cytology >Sequential Assembly of Myosin II an IQGAP-like Protein and Filamentous Actin to a Ring Structure Involved in Budding Yeast Cytokinesis
【2h】

Sequential Assembly of Myosin II an IQGAP-like Protein and Filamentous Actin to a Ring Structure Involved in Budding Yeast Cytokinesis

机译：肌球蛋白IIIQGAP样蛋白和丝状肌动蛋白的顺序组装到涉及酵母胞质分裂的环结构。

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

We have identified a Saccharomyces cerevisiae protein, Cyk1p, that exhibits sequence similarity to the mammalian IQGAPs. Gene disruption of Cyk1p results in a failure in cytokinesis without affecting other events in the cell cycle. Cyk1p is diffused throughout most of the cell cycle but localizes to a ring structure at the mother–bud junction after the initiation of anaphase. This ring contains filamentous actin and Myo1p, a myosin II homologue. In vivo observation with green fluorescent protein–tagged Myo1p showed that the ring decreases drastically in size during cell division and therefore may be contractile. These results indicate that cytokinesis in budding yeast is likely to involve an actomyosin-based contractile ring. The assembly of this ring occurs in temporally distinct steps: Myo1p localizes to a ring that overlaps the septins at the G1-S transition slightly before bud emergence; Cyk1p and actin then accumulate in this ring after the activation of the Cdc15 pathway late in mitosis. The localization of myosin is abolished by a mutation in Cdc12p, implicating a role for the septin filaments in the assembly of the actomyosin ring. The accumulation of actin in the cytokinetic ring was not observed in cells depleted of Cyk1p, suggesting that Cyk1p plays a role in the recruitment of actin filaments, perhaps through a filament-binding activity similar to that demonstrated for mammalian IQGAPs.
机译：我们已经鉴定出一种酿酒酵母蛋白Cyk1p，它与哺乳动物IQGAPs表现出序列相似性。 Cyk1p的基因破坏导致胞质分裂失败，而不影响细胞周期中的其他事件。 Cyk1p扩散到整个细胞周期的大部分时间，但在后期开始后定位于母芽连接处的环状结构。该环包含丝状肌动蛋白和Myo1p，它是肌球蛋白II的同源物。用绿色荧光蛋白标记的Myo1p进行的体内观察显示，该环在细胞分裂过程中尺寸急剧减小，因此可能会收缩。这些结果表明，发芽酵母中的胞质分裂可能涉及基于肌动球蛋白的收缩环。这个环的组装发生在时间上不同的步骤中：Myo1p定位于一个环，该环在芽出现之前的G1-S过渡期与septins重叠；在有丝分裂后期激活Cdc15途径后，Cyk1p和肌动蛋白在该环中积累。 Cdc12p中的突变消除了肌球蛋白的定位，这暗示着隔肌丝在肌动球蛋白环组装中的作用。在耗尽Cyk1p的细胞中未观察到肌动蛋白在细胞动力学环中的积累，这表明Cyk1p可能在肌动蛋白丝的募集中发挥了作用，也许是通过与哺乳动物IQGAP相似的细丝结合活性。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号