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首页> 美国卫生研究院文献>Journal of the Boston Society of Medical Sciences >Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates
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Detection of a Sulfotransferase (HEC-GlcNAc6ST) in High Endothelial Venules of Lymph Nodes and in High Endothelial Venule-Like Vessels within Ectopic Lymphoid Aggregates

机译：淋巴结的高内皮小静脉和异位淋巴聚集物中的高内皮小静脉样血管中的硫转移酶（HEC-GlcNAc6ST）的检测。

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The interaction of L-selectin on lymphocytes with sulfated ligands on high endothelial venules (HEVs) of lymph nodes results in lymphocyte rolling and is essential for lymphocyte homing. The MECA-79 monoclonal antibody reports HEV-expressed ligands for L-selectin by recognizing a critical sulfation-dependent determinant on these ligands. HEC-GlcNAc6ST, a HEV-localized sulfotransferase, is essential for the elaboration of functional ligands within lymph nodes, as well as the generation of the MECA-79 epitope. Here, we use an antibody against murine HEC-GlcNAc6ST to study its expression in relationship to the MECA-79 epitope. In lymph nodes, the enzyme is expressed in the Golgi apparatus of high endothelial cells, in close correspondence with luminal staining by MECA-79. In lymph node HEVs of HEC-GlcNAc6ST-null mice, luminal staining by MECA-79 is almost abolished, whereas abluminal staining persists although reduced in intensity. HEV-like vessels in several examples of inflammation-associated lymphoid neogenesis, including nonobese diabetic mice, also exhibit concomitant expression of the sulfotransferase and luminal MECA-79 reactivity. The correlation extends to ectopic lymphoid aggregates within the pancreas of RIP-BLC mice, in which CXCL13 is expressed in islets. Analysis of the progeny of RIP-BLC by HEC-GlcNAc6ST-null mice establishes that the enzyme is responsible for the MECA-79 defined luminal ligands.
机译：淋巴细胞上的L-选择蛋白与淋巴结的高内皮小静脉（HEV）上的硫酸化配体的相互作用导致淋巴细胞滚动，这对于淋巴细胞归巢是必不可少的。 MECA-79单克隆抗体通过识别这些配体上关键的硫酸盐依赖性决定簇，报道了HEV表达的L-选择蛋白配体。 HEC-GlcNAc6ST是HEV定位的磺基转移酶，对于在淋巴结内拟定功能性配体以及生成MECA-79表位至关重要。在这里，我们使用抗鼠HEC-GlcNAc6ST的抗体来研究其与MECA-79表位的关系。在淋巴结中，该酶在高内皮细胞的高尔基体中表达，与MECA-79的腔内染色密切相关。在HEC-GlcNAc6ST-null小鼠的淋巴结HEV中，MECA-79的腔内染色几乎被废除了，尽管强度降低了，但腔内染色仍然存在。在一些与炎症相关的淋巴新生过程中，包括非肥胖型糖尿病小鼠，HEV样血管也表现出磺基转移酶和管腔MECA-79反应性的同时表达。相关性扩展到RIP-BLC小鼠胰腺内的异位淋巴样聚集体，其中CXCL13在胰岛中表达。通过HEC-GlcNAc6ST-null小鼠对RIP-BLC的后代进行分析，确定该酶负责MECA-79定义的腔配体。

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