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首页> 美国卫生研究院文献>Journal of the Boston Society of Medical Sciences >Characterization of intestinal brush border cytoskeletal proteins of normal and neoplastic human epithelial cells. A comparison with the avian brush border.
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Characterization of intestinal brush border cytoskeletal proteins of normal and neoplastic human epithelial cells. A comparison with the avian brush border.

机译：正常和赘生性人类上皮细胞的肠刷缘细胞骨架蛋白的表征。与鸟类画笔边框的比较。

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摘要

The elaborate cytoskeletal matrix underlying the intestinal epithelial cell brush border (BB) is the hallmark of a mature enterocyte. As such, alterations in this structure are potentially useful as markers aiding in the recognition of subtle defects in cell maturation, such as those accompanying dysplasia and neoplasia. For exploration of this hypothesis, the BB components of human ileal and colonic enterocytes have been compared structurally and biochemically with the well-characterized avian BB, and alterations in the BB cytoskeleton in various states of dysplasia and neoplasia have been identified. Ultrastructural analysis of isolated human ileal BBs indicate that the human BB is structurally homologous to BBs isolated from chicken and other mammalian sources. Like other mammalian BBs (eg, from rat) the terminal web cytoskeleton of the human BB is less extensive than that in the avian BB. Immunochemical analysis of isolated human BBs indicates that the major proteins of the avian microvillar actin bundle, villin, fimbrin, and the 110-kd subunit of the 110K-calmodulin complex, are all present in the human BB. The terminal web protein myosin is also present. Unlike the terminal web of the avian BB, which contains a BB-specific isoform of spectrin, TW 260/240, the human BB contains the more widely distributed spectrin isoform, fodrin. In addition, the human BB contains multiple proteins immunoreactive with antibodies to protein 4.1, a spectrin/actin binding protein that is absent from the avian BB. Immunolocalization studies examining the distribution of the BB-specific microvillar protein, villin, in human colonic mucosa indicate that the localization of this protein is disrupted in certain dysplastic and neoplastic states. Thus, both the expression and/or distribution of BB-specific proteins such as villin may be useful markers for defects in the differentiation state of the enterocyte.
机译：肠上皮细胞刷状边界（BB）下方的精细细胞骨架基质是成熟肠细胞的标志。因此，这种结构的改变可用作标记物，有助于识别细胞成熟中的细微缺陷，例如伴随不典型增生和瘤形成的那些。为了探索这一假设，已将人类回肠和结肠肠上皮细胞的BB成分与特征明确的禽BB进行了结构和生化比较，并且已鉴定出在异型增生和瘤形成的各种状态下BB细胞骨架的变化。分离的人回肠BB的超微结构分析表明，人BB与从鸡和其他哺乳动物来源分离的BB在结构上是同源的。像其他哺乳动物BB（例如，来自大鼠）一样，人BB的末端网状细胞骨架不如禽BB广泛。分离的人BB的免疫化学分析表明，禽微微肌动蛋白束的主要蛋白质，维尔林，纤维蛋白和110K-钙调蛋白复合物的110-kd亚基都存在于人BB中。末端网蛋白肌球蛋白也存在。不同于禽BB的末端网，其包含血红蛋白的BB特异性同工型，TW 260/240，人类BB含有分布更广的血红蛋白同工型，fodrin。此外，人BB包含多种与蛋白4.1抗体发生免疫反应的蛋白，该蛋白是禽BB所不具备的血影蛋白/肌动蛋白结合蛋白。免疫定位研究检查了BB特异性微绒毛蛋白villin在人结肠粘膜中的分布，表明该蛋白的定位在某些发育异常和赘生状态下被破坏。因此，BB特异性蛋白例如villin的表达和/或分布可能是肠细胞分化状态缺陷的有用标记。

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