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首页> 美国卫生研究院文献>Journal of the Boston Society of Medical Sciences >Expression of the Vascular Endothelial Growth Factor C Receptor VEGFR-3 in Lymphatic Endothelium of the Skin and in Vascular Tumors
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Expression of the Vascular Endothelial Growth Factor C Receptor VEGFR-3 in Lymphatic Endothelium of the Skin and in Vascular Tumors

机译：血管内皮生长因子C受体VEGFR-3在皮肤淋巴管内皮细胞和血管肿瘤中的表达

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摘要

It is difficult to identify lymph vessels in tissue sections by histochemical staining, and thus a specific marker for lymphatic endothelial cells would be more practical in histopathological diagnostics. Here we have applied a specific antigenic marker for lymphatic endothelial cells in the human skin, the vascular endothelial growth factor receptor-3 (VEGFR-3), and show that it identifies a distinct vessel population both in fetal and adult skin, which has properties of lymphatic vessels. The expression of VEGFR-3 was studied in normal human skin by in situ hybridization, iodinated ligand binding, and immunohistochemistry. A subset of developing vessels expressed the VEGFR-3 mRNA in fetal skin as shown by in situ hybridization and radioiodinated vascular endothelial growth factor (VEGF)-C bound selectively to a subset of vessels in adult skin that had morphological characteristics of lymphatic vessels. Monoclonal antibodies against the extracellular domain of VEGFR-3 stained specifically endothelial cells of dermal lymph vessels, in contrast to PAL-E antibodies, which stained only blood vessel endothelia. In addition, staining for VEGFR-3 was strongly positive in the endothelium of cutaneous lymphangiomatosis, but staining of endothelial cells in cutaneous hemangiomas was weaker. These results establish the utility of anti-VEGFR-3 antibodies in the identification of lymphovascular channels in the skin and in the differential diagnosis of skin lesions involving lymphatic or blood vascular endothelium.
机译：通过组织化学染色难以识别组织切片中的淋巴管，因此淋巴管内皮细胞的特异性标记物在组织病理学诊断中将更为实用。在这里，我们为人类皮肤中的淋巴管内皮细胞应用了一种特定的抗原标记，即血管内皮生长因子受体3（VEGFR-3），并显示出它可以识别胎儿和成年皮肤中不同的血管群，具有一定的特性淋巴管。通过原位杂交，碘配体结合和免疫组织化学研究了正常人皮肤中VEGFR-3的表达。如原位杂交所示，一部分发育中的血管在胎儿皮肤中表达VEGFR-3 mRNA，放射性碘标记的血管内皮生长因子（VEGF）-C选择性结合至成年皮肤中一部分具有淋巴管形态特征的血管。与PAL-E抗体（仅染色血管内皮细胞）相反，针对VEGFR-3胞外域的单克隆抗体可特异性染色真皮淋巴管的内皮细胞。另外，VEGFR-3的染色在皮肤淋巴管瘤病的内皮中强烈阳性，但是在皮肤血管瘤中的内皮细胞染色较弱。这些结果确立了抗VEGFR-3抗体在鉴定皮肤中的淋巴血管通道和在涉及淋巴或血管内皮的皮肤损伤的鉴别诊断中的用途。

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