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首页> 美国卫生研究院文献>Journal of Clinical Microbiology >Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.
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Identification of Escherichia coli that produces heat-stable enterotoxin STA by a commercially available enzyme-linked immunoassay and comparison of the assay with infant mouse and DNA probe tests.

机译：通过可商购的酶联免疫测定法鉴定产生热稳定肠毒素STA的大肠杆菌，并与婴儿小鼠和DNA探针测试进行比较。

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摘要

By using the infant mouse test and a commercially available competitive enzyme-linked immunosorbent assay (ELISA), 100 strains of Escherichia coli carrying STA1 or STA2 genes were shown to produce the heat-stable enterotoxin STA. An additional 100 strains were negative in both tests. The ELISA was easy to perform, and results were available within 24 h. Testing strains with an enzyme-linked DNA probe kit that incorporated both STA1- and STA2-specific oligonucleotides showed that the 100 strains positive in the mouse test and ELISA also hybridized with the mixed probe. Two strains carrying STA1 genes but negative in the mouse test and ELISA also hybridized with the mixed probe.
机译：通过使用婴儿小鼠试验和可商购的竞争性酶联免疫吸附测定（ELISA），显示了100个带有STA1或STA2基因的大肠杆菌菌株产生了热稳定肠毒素STA。在两个测试中另外100个菌株均为阴性。 ELISA易于执行，并且可在24小时内获得结果。用掺有STA1和STA2特异性寡核苷酸的酶联DNA探针试剂盒测试菌株，结果表明，在小鼠测试和ELISA中呈阳性的100个菌株也与混合探针杂交。携带STA1基因但在小鼠试验和ELISA中呈阴性的两种菌株也与混合探针杂交。

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