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首页> 美国卫生研究院文献>Micromachines >Mechanophenotyping of B16 Melanoma Cell Variants for the Assessment of the Efficacy of (-)-Epigallocatechin Gallate Treatment Using a Tapered Microfluidic Device
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Mechanophenotyping of B16 Melanoma Cell Variants for the Assessment of the Efficacy of (-)-Epigallocatechin Gallate Treatment Using a Tapered Microfluidic Device

机译：B16黑色素瘤细胞变体的机械表型评估使用锥形微流控设备的（-）-表没食子儿茶素没食子酸酯治疗功效的评估

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摘要

Metastatic cancer cells are known to have a smaller cell stiffness than healthy cells because the small stiffness is beneficial for passing through the extracellular matrix when the cancer cells instigate a metastatic process. Here we developed a simple and handy microfluidic system to assess metastatic capacity of the cancer cells from a mechanical point of view. A tapered microchannel was devised through which a cell was compressed while passing. Two metastasis B16 melanoma variants (B16-F1 and B16-F10) were examined. The shape recovery process of the cell from a compressed state was evaluated with the Kelvin–Voigt model. The results demonstrated that the B16-F10 cells showed a larger time constant of shape recovery than B16-F1 cells, although no significant difference in the initial strain was observed between B16-F1 cells and B16-F10 cells. We further investigated effects of catechin on the cell deformability and found that the deformability of B16-F10 cells was significantly decreased and became equivalent to that of untreated B16-F1 cells. These results addressed the utility of the present system to handily but roughly assess the metastatic capacity of cancer cells and to investigate drug efficacy on the metastatic capacity.
机译：已知转移的癌细胞具有比健康细胞小的细胞刚度，因为当癌细胞激发转移过程时，小的刚度有利于穿过细胞外基质。在这里，我们开发了一种简单方便的微流体系统，从机械角度评估癌细胞的转移能力。设计了锥形微通道，使细胞通过时被压缩。检查了两个转移性B16黑色素瘤变体（B16-F1和B16-F10）。使用Kelvin-Voigt模型评估了细胞从压缩状态恢复的形状。结果表明，尽管在B16-F1细胞和B16-F10细胞之间未观察到初始应变的显着差异，但B16-F10细胞显示出比B16-F1细胞更大的形状恢复时间常数。我们进一步研究了儿茶素对细胞变形能力的影响，发现B16-F10细胞的变形能力显着降低，并变得与未处理的B16-F1细胞相当。这些结果解决了本系统在方便但粗略地评估癌细胞的转移能力以及研究对转移能力的药物功效方面的实用性。

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