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首页> 美国卫生研究院文献>Nucleic Acids Research >Supragenic loop organization: mapping in Drosophila embryos, of scaffold-associated regions on a 800 kilobase DNA continuum cloned from the 14B-15B first chromosome region.
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Supragenic loop organization: mapping in Drosophila embryos, of scaffold-associated regions on a 800 kilobase DNA continuum cloned from the 14B-15B first chromosome region.

机译：超基因环组织：在果蝇胚胎中，从14B-15B第一个染色体区域克隆的800千碱基DNA连续体上与支架相关的区域作图。

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The supragenic loop organization of the Drosophila genome was investigated on a 800 kilobase (kb) DNA continuum from the 14B-15B first chromosome region. Nuclear scaffolds from 0-18 hr embryos were prepared with Laemmli's low-salt, detergent procedure and digested with restriction enzymes. Scaffold-associated regions (SARs) were mapped by probing Southern transfers of total, scaffold-associated and free DNA with a set of 70 recombinant phages overlapping the investigated genomic region. In all, 85 restriction fragments showed association to scaffolds. 12 of them were present in the majority of scaffolds. They bore strong SARs organizing the DNA molecule as consecutive loops with sizes ranging from 15 to 115 kb. 44 were present in only a fraction of scaffolds. They contained weak SARs subdividing the basic loops into smaller ones. 29 additional restriction fragments were present in a very small fraction of scaffolds. The position of SARs with respect to transcribed regions was investigated. Strong SARs appeared to be located on untranscribed DNA and to frame transcription units. In contrast, at least some weak SARs were shown to comap with transcribed regions or to reside within characterized transcription units. Statistical analyses established that strong and weak SARs were periodically positioned on the DNA continuum and that there was a potential contact point between scaffolds and the DNA continuum every 11 kb, or multiples thereof. Implications for SAR role(s) are discussed.
机译：果蝇基因组的超基因环组织是在来自14B-15B第一染色体区域的800 kb（kb）DNA连续体上进行研究的。用Laemmli的低盐，去垢程序制备0-18小时胚胎的核支架，并用限制酶消化。支架相关区域（SARs）通过探测70个重组噬菌体与研究基因组区域重叠的总DNA，支架相关和游离DNA的Southern转移来作图。共有85个限制性片段显示与支架相关。其中12个存在于大多数支架中。它们带有强大的SAR，将DNA分子组织成连续的环，大小从15到115 kb不等。仅一部分支架中存在44个。它们包含较弱的SAR，将基本回路细分为较小的回路。在非常小的支架中还存在29个其他限制性片段。研究了SARs相对于转录区的位置。强SAR似乎位于未转录的DNA上并构筑转录单位。相反，显示出至少一些弱SAR与转录区共图或位于特征转录单位内。统计分析确定，强和弱SAR会定期定位在DNA连续体上，并且每11 kb或其倍数，支架和DNA连续体之间存在潜在的接触点。讨论了对SAR角色的影响。

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