• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Nutrients >Long-Term Impact of Neonatal Intake of Oleanolic Acid on the Expression of AMP-Activated Protein Kinase, Adiponectin and Inflammatory Cytokines in Rats Fed with a High Fructose Diet
【2h】

Long-Term Impact of Neonatal Intake of Oleanolic Acid on the Expression of AMP-Activated Protein Kinase, Adiponectin and Inflammatory Cytokines in Rats Fed with a High Fructose Diet

机译：新生儿摄入齐墩果酸对高果糖饮食喂养的大鼠中AMP活化蛋白激酶，脂联素和炎性细胞因子表达的长期影响

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

AMP-activated protein kinase (AMPK) is known to regulate both glucose and lipid metabolism, which play vital roles in the development of metabolic syndrome. One way of regulating AMPK is through hormonal activation using adiponectin. Patients diagnosed with type-2 diabetes (T2D) and obesity exhibit low adiponectin concentration levels in their blood. Moreover, studies have also shown that inflammatory processes play a significant role in the etiology of these metabolic diseases. In this study, the long-term effects of neonatal intake of oleanolic acid (OA) on the AMPK gene, genes associated with glucose transport and lipid metabolism, adiponectin levels, and inflammatory biomarkers in rats fed with a high fructose diet were investigated. Seven day old pups were randomly divided into five groups and treated as follows; 0.5% dimethylsulphoxide v/v in distilled water vehicle control (CON), oleanolic acid (OA, 60 mg/kg), high fructose diet (HF, 20% w/v), high fructose diet combined with oleanolic acid (HF+OA), and high fructose diet combined with metformin (HF+MET, 500 mg/kg). The treatments were administered once daily until day 14. The rats were then weaned at day 21 and fed standard rat chow and had ad libitum access to plain drinking water until day 112. The quantitative polymerase chain reaction (qPCR) was used to analyze the gene expressions of AMPK, Glut-4, Cpt-1, AdipoR1, AdipoR2, TNF-α, and IL-6 in the skeletal muscles. Bio-Plex Pro magnetic bead-based assay was used to measure plasma levels of inflammatory markers (TNF-α, IL-6, VEGF, and MCP-1) while ELISA kits were used to measure adiponectin concentration in blood plasma. The results obtained in this study showed that neonatal supplementation with OA significantly increased AMPK gene expression approximately ~4-fold in OA fed rats compared to those that were fed with HF alone. In addition, glut-4 gene expression was also significantly higher in the OA treatment group compared to all the other experimental groups except the CON group whereas Cpt-1 gene was more expressed when OA was administered alone. Together, these results indicated that OA can play a role in glucose and lipid metabolism gene regulation. Furthermore, the results showed that the OA group had ~1.5-fold increase in adiponectin concentration when comparedto the HF group. Moreover, HF increased levels of inflammatory cytokines, which was attenuated by neonatal administration of OA. Plasma concentration and gene expression in the skeletal muscle for TNF-α and IL-6 were significantly increased in rats that were treated with HF alone when compared to all the other groups. On the contrary, the high levels of TNF-α and IL-6 were reduced when OA was administered. These findings suggest that intake of oleanolic acid during the neonatal stage of development could be a potential strategic intervention for the long-term prevention of metabolic diseases such as T2D and obesity.
机译：已知AMP激活的蛋白激酶（AMPK）调节葡萄糖和脂质代谢，这在代谢综合征的发展中起着至关重要的作用。调节AMPK的一种方法是使用脂联素通过激素激活。被诊断患有2型糖尿病（T2D）和肥胖的患者血液中脂联素浓度较低。此外，研究还表明，炎症过程在这些代谢疾病的病因中起着重要作用。在这项研究中，调查了高果糖饮食喂养的大鼠中新生儿摄入齐墩果酸（OA）对AMPK基因，与葡萄糖转运和脂质代谢相关的基因，脂联素水平以及炎症生物标志物的长期影响。将7日龄的幼犬随机分为5组，并按以下方法处理：蒸馏水赋形剂对照（CON）中0.5％的二甲基亚砜v / v，齐墩果酸（OA，60 mg / kg），高果糖饮食（HF，20％w / v），高果糖饮食与齐墩果酸（HF + OA） ）和高果糖饮食加二甲双胍（HF + MET，500 mg / kg）。每天进行一次治疗，直到第14天。然后在第21天给大鼠断奶，喂食标准大鼠食物，直到第112天都可以自由饮水。使用定量聚合酶链反应（qPCR）分析该基因骨骼肌中AMPK，Glut-4，Cpt-1，AdipoR1，AdipoR2，TNF-α和IL-6的表达使用基于Bio-Plex Pro磁珠的测定法来测量血浆中炎症标志物（TNF-α，IL-6，VEGF和MCP-1）的水平，而使用ELISA试剂盒来测定血浆中脂联素的浓度。在这项研究中获得的结果表明，与单独饲喂HF的大鼠相比，在OA饲喂的大鼠中，新生儿补充OA可以显着提高AMPK基因表达约4倍。另外，除CON组外，OA治疗组中的glut-4基因表达也显着高于所有其他实验组，而单独给予OA时，Cpt-1基因表达更多。总之，这些结果表明OA可以在葡萄糖和脂质代谢基因的调控中起作用。此外，结果表明，与HF组相比，OA组的脂联素浓度增加了约1.5倍。而且，HF增加了炎性细胞因子的水平，这通过新生儿给予OA而减弱。与其他所有组相比，单独用HF治疗的大鼠的TNF-α和IL-6的骨骼肌血浆浓度和基因表达显着增加。相反，施用OA可降低高水平的TNF-α和IL-6。这些发现表明，在新生儿发育阶段摄入齐墩果酸可能是长期预防代谢性疾病（如T2D和肥胖）的潜在战略干预措施。

著录项

相似文献

  • 外文文献
  • 中文文献
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号