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Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as a paradigm

机译：简单而严格的筛选方法用于评估假定的转化子对非生物胁迫的耐受性：盐和镉胁迫为范例

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摘要

Rigorous and stringent screening methodologies to select transformants at both seedling and plant level under cadmium or NaCl stress were developed. At seedling level, two screening strategies were standardized. One involved germination on filter paper/agar in the presence of either CdCl2 (125 μM) or NaCl (350–450 mM) for 9 days and selection of tolerant putative transformants. The other involved germination of the seedlings on soilrite by irrigation of 450 mM NaCl. Further, at plant level, in vitro evaluation for stress tolerance involved a simple leaf senescence bioassay. Combination of the seedling and plant level screening strategies would result in the initial identification of promising transformants for further analysis.

机译：建立了严格和严格的筛选方法，以选择镉或NaCl胁迫下的幼苗和植物水平的转化子。在苗期，标准化了两种筛选策略。一种方法是在CdCl2（125μM）或NaCl（350-450 mM）存在下在滤纸/琼脂上萌发9天，然后选择耐受的推定转化子。另一个涉及通过灌溉450 mM NaCl在土壤土上发芽的种子。此外，在植物水平上，体外抗逆性评估涉及简单的叶片衰老生物测定。结合幼苗水平和植物水平筛选策略将初步鉴定出有希望的转化子，以供进一步分析。

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期刊名称 Physiology and Molecular Biology of Plants
作者
Sushma M. Awaji; V. Nagaveni; Prashantkumar; S. Hanjagi; D. N. Madhvi; V. R. Sashidhar; Rohini Sreevathsa;
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作者单位

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年(卷),期 2010(16),2
年度 2010
页码 115–121
总页数 7
原文格式 PDF
正文语种
中图分类分子生物学;
关键词
Abiotic stress evaluation methodology transgenics;

机译：非生物胁迫;评估;方法;转基因;

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1. Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as a paradigm [J] . Sushma M. Awaji, V. Nagaveni, Prashantkumar, Physiology and Molecular Biology of Plants . 2010,第2期

机译：简单而严格的筛选方法，用于评估假定的转化子对非生物胁迫的耐受性：盐和镉胁迫为范例
2. Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as a paradigm [J] . Awaji Sushma M., Nagaveni V., Prashantkumar, Physiology and Molecular Biology of Plants . 2010,第2期

机译：简单而严格的筛选方法，用于评估推定的转化子对非生物胁迫的耐受性：盐和镉胁迫为范例
3. In Vitro Screening and Identification of Putative Sunflower {Helianthus annus L.) Transformants Expressing ECNAC1 Gene by Salt Stress Method [J] . K.C. MANJUNATH, A. MAHADEVA, ROHINI SREEVATHSA, Trends in Biosciences . 2013,第1期

机译：盐胁迫法体外筛选表达ECNAC1基因的向日葵（Helianthus annus L.）转化体
4. Metabolomic exploration of abiotic stress tolerance of stress-tolerant plants [C] . Charles R. Warren American Society for Mass Spectrometry Conference on Mass Spectrometry and Allied Topics . 2010

机译：应力耐受植物的非生物胁迫耐受性的代谢组探索
5. Screening for abiotic stress tolerance in abscisic acid-insensitive wheat (Triticum aestivum L.) [D] . Farooqi, Shakeelur Rahman 1994

机译：脱落酸不敏感小麦（Triticum aestivum L.）非生物胁迫耐受性的筛选
6. Suppression of Melatonin 2-Hydroxylase Increases Melatonin Production Leading to the Enhanced Abiotic Stress Tolerance against Cadmium Senescence Salt and Tunicamycin in Rice Plants [O] . Geun-Hee Choi, Kyoungwhan Back 2019

机译：抑制褪黑激素2-羟化酶可增加褪黑激素的产生从而导致水稻植物对镉衰老盐和衣霉素的非生物胁迫耐受性增强。
7. Stress Tolerance Profiling of a Collection of Extant Salt-Tolerant Rice Varieties and Transgenic Plants Overexpressing Abiotic Stress Tolerance Genes [O] . Ken-ichi Kurotani, Kazumasa Yamanaka, Yosuke Toda, 2015

机译：耐盐耐盐水稻品种集合的应力耐受性和过表达非反应性胁迫耐受基因的转基因植物

Simple yet stringent screening methodologies for evaluation of putative transformants for abiotic stress tolerance: salt and cadmium stress as a paradigm

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