首页> 美国卫生研究院文献>PLoS Clinical Trials >Ecomorph or Endangered Coral? DNA and Microstructure Reveal Hawaiian Species Complexes: Montipora dilatata/flabellata/turgescens M. patula/verrilli
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Ecomorph or Endangered Coral? DNA and Microstructure Reveal Hawaiian Species Complexes: Montipora dilatata/flabellata/turgescens M. patula/verrilli

机译:生态形态还是濒临灭绝的珊瑚? DNA和微结构揭示了夏威夷物种的复合体:蒙蒂帕拉(Montipora dilatata)/ flabellata / turgescens和摩提摩M(M. patula)/ verrilli

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摘要

M. dilatata, M. flabellata, and M. patula and 80 other scleractinian corals were petitioned to be listed under the US Endangered Species Act (ESA), which would have major conservation implications. One of the difficulties with this evaluation is that reproductive boundaries between morphologically defined coral species are often permeable, and morphology can be wildly variable. We examined genetic and morphological variation in Hawaiian Montipora with a suite of molecular markers (mitochondrial: COI, CR, Cyt-B, 16S, ATP6; nuclear: ATPsβ, ITS) and microscopic skeletal measurements. Mitochondrial markers and the ITS region revealed four distinct clades: I) M. patula/M. verrilli, II) M. cf. incrassata, III) M. capitata, IV) M. dilatata/M. flabellata/M. cf. turgescens. These clades are likely to occur outside of Hawai'i according to mitochondrial control region haplotypes from previous studies. The ATPsβ intron data showed a pattern often interpreted as resulting from hybridization and introgression; however, incomplete lineage sorting may be more likely since the multicopy nuclear ITS region was consistent with the mitochondrial data. Furthermore, principal components analysis (PCA) of skeletal microstructure was concordant with the mitochondrial clades, while nominal taxa overlapped. The size and shape of verrucae or papillae contributed most to identifying groups, while colony-level morphology was highly variable. It is not yet clear if these species complexes represent population-level variation or incipient speciation (CA<1MYA), two alternatives that have very different conservation implications. This study highlights the difficulty in understanding the scale of genetic and morphological variation that corresponds to species as opposed to population-level variation, information that is essential for conservation and for understanding coral biodiversity.
机译:美国要求将濒危物种(ESA)列入M. dilatata,M。flabellata和M. patula以及80种其他Scleractinian珊瑚的名单。这种评估的困难之一是形态界定的珊瑚物种之间的繁殖界限通常是可渗透的,形态可能会发生巨大变化。我们用一套分子标记物(线粒体:COI,CR,Cyt-B,16S,ATP6;核仁:ATPsβ,ITS)和显微骨骼学检查检查了夏威夷Montipora的遗传和形态变异。线粒体标记和ITS区域显示四个不同的进化枝:I)pat./M。patula / M。 verrilli,II)M. cf. incrassata,III)capitata,IV)di。 flabellata / M。 cf. turgescens。根据先前研究的线粒体控制区单倍型,这些进化枝很可能发生在夏威夷之外。 ATPsβ内含子数据显示出一种通常被解释为杂交和渗入的模式。然而,由于多核ITS区域与线粒体数据一致,因此谱系排序不完全的可能性更高。此外,骨骼微结构的主成分分析(PCA)与线粒体进化枝一致,而名义分类群重叠。疣状或乳头状的大小和形状最有助于鉴定群体,而菌落水平的形态变化很大。目前尚不清楚这些物种复合物是否代表种群水平的变异或初期物种形成(CA <1MYA),这是两种具有不同保护意义的替代方案。这项研究突显了难以理解与物种相对应的遗传和形态变异的规模,而不是种群水平的变异,这对于保护和理解珊瑚生物多样性至关重要。

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