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首页> 美国卫生研究院文献>PLoS Clinical Trials >Functional Divergence among Silkworm Antimicrobial Peptide Paralogs by the Activities of Recombinant Proteins and the Induced Expression Profiles
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Functional Divergence among Silkworm Antimicrobial Peptide Paralogs by the Activities of Recombinant Proteins and the Induced Expression Profiles

机译：重组蛋白的活性和诱导的表达谱之间的家蚕抗菌肽旁系同源物的功能分歧。

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Antimicrobial peptides are small-molecule proteins that are usually encoded by multiple-gene families. They play crucial roles in the innate immune response, but reports on the functional divergence of antimicrobial peptide gene families are rare. In this study, 14 paralogs of antimicrobial peptides belonging to cecropin, moricin and gloverin families were recombinantly expressed in pET expression systems. By antimicrobial activity tests, peptides representing paralogs in the same family of cecropin and moricin families, displayed remarkable differences against 10 tested bacteria. The evolutionary rates were relatively fast in the two families, which presented obvious functional divergence among paralogs of each family. Four peptides of gloverin family had similar antimicrobial spectrum and activity against tested bacteria. The gloverin family showed similar antimicrobial function and slow evolutionary rates. By induced transcriptional activity, genes encoding active antimicrobial peptides were upregulated at obviously different levels when silkworm pupae were infected by three types of microbes. Association analysis of antimicrobial activities and induced transcriptional activities indicated that the antimicrobial activities might be positively correlated with induced transcriptional activities in the cecropin and moricin families. These results suggest that representative BmcecB6, BmcecD and Bmmor as the major effector genes have broad antimicrobial spectrum, strong antimicrobial activity and high microbe-induced expression among each family and maybe play crucial roles in eliminating microbial infection.
机译：抗菌肽是通常由多基因家族编码的小分子蛋白质。它们在先天免疫应答中起关键作用，但是有关抗菌肽基因家族功能差异的报道很少。在这项研究中，属于天蚕素，莫里菌素和手套蛋白家族的14种抗菌肽的旁系同源物在pET表达系统中重组表达。通过抗菌活性测试，代表天蚕素和moricin家族同一家族的旁系同源物的肽对10种被测试细菌表现出显着差异。两个家族的进化速度相对较快，这在每个家族的旁系同源物之间表现出明显的功能差异。手套蛋白家族的四种肽具有相似的抗菌谱和对被测细菌的活性。手套蛋白家族显示出相似的抗菌功能，并且进化速度较慢。通过诱导转录活性，当蚕silk被三种类型的微生物感染时，编码活性抗菌肽的基因以明显不同的水平上调。抗菌活性和诱导的转录活性的关联分析表明，抗菌活性可能与天蚕素和moricin家族的诱导转录活性呈正相关。这些结果表明，代表性的BmcecB6，BmcecD和Bmmor作为主要的效应基因具有广泛的抗菌谱，强大的抗菌活性和较高的微生物诱导表达水平，并且可能在消除微生物感染中起关键作用。

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