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首页> 美国卫生研究院文献>PLoS Clinical Trials >Coordinated miRNA/mRNA Expression Profiles for Understanding Breed-Specific Metabolic Characters of Liver between Erhualian and Large White Pigs
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Coordinated miRNA/mRNA Expression Profiles for Understanding Breed-Specific Metabolic Characters of Liver between Erhualian and Large White Pigs

机译：协调的miRNA / mRNA表达谱，用于了解二花脸和大型白猪之间肝脏特定代谢的特性

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MicroRNAs (miRNAs) are involved in the regulation of various metabolic processes in the liver, yet little is known on the breed-specific expression profiles of miRNAs in coordination with those of mRNAs. Here we used two breeds of male newborn piglets with distinct metabolic characteristics, Large White (LW) and Erhualian (EHL), to delineate the hepatic expression profiles of mRNA with microarray and miRNAs with both deep sequencing and microarray, and to analyze the functional relevance of integrated miRNA and mRNA expression in relation to the physiological and biochemical parameters. EHL had significantly lower body weight and liver weight at birth, but showed elevated serum levels of total cholesterol (TCH), high-density lipoprotein cholesterol (HDLC) and low-density lipoprotein cholesterol (LDLC), as well as higher liver content of cholesterol. Higher serum cortisol and lower serum insulin and leptin were also observed in EHL piglets. Compared to LW, 30 up-regulated and 18 down-regulated miRNAs were identified in the liver of EHL, together with 298 up-regulated and 510 down-regulated mRNAs (FDR<10%). RT-PCR validation of some differentially expressed miRNAs (DEMs) further confirmed the high-throughput data analysis. Using a target prediction algorithm, we found significant correlation between the up-regulated miRNAs and down-regulated mRNAs. Moreover, differentially expressed genes (DEGs), which are involved in proteolysis, were predicted to be mediated by DEMs. These findings provide new information on the miRNA and mRNA profiles in porcine liver, which would shed light on the molecular mechanisms underlying the breed-specific traits in the pig, and may serve as a basis for further investigation into the biological functions of miRNAs in porcine liver.
机译：微小RNA（miRNA）参与肝脏中各种代谢过程的调节，但与mRNA协同作用的miRNA的品种特异性表达谱知之甚少。在这里，我们使用了具有不同代谢特征的两种雄性新生仔猪，大白（LW）和二化lian（EHL），通过微阵列和深测序和微阵列描绘了mRNA在肝脏中的表达谱，并分析了功能相关性的miRNA和mRNA表达与生理和生化参数的关系。 EHL出生时体重和肝脏重量显着降低，但血清总胆固醇（TCH），高密度脂蛋白胆固醇（HDLC）和低密度脂蛋白胆固醇（LDLC）升高，肝脏中胆固醇含量更高。在EHL仔猪中还观察到较高的血清皮质醇和较低的胰岛素和瘦素。与LW相比，在EHL肝脏中鉴定出30个上调的miRNA和18个下调的miRNA，以及298个上调和510个下调的mRNA（FDR <10％）。一些差异表达的miRNA（DEM）的RT-PCR验证进一步证实了高通量数据分析。使用目标预测算法，我们发现上调的miRNA和下调的mRNA之间存在显着相关性。此外，参与蛋白水解的差异表达基因（DEGs）预计由DEM介导。这些发现为猪肝脏中的miRNA和mRNA谱提供了新的信息，这将为猪的特定品种性状的潜在分子机制提供线索，并可能为进一步研究猪中miRNA的生物学功能提供基础。肝。

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