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首页> 美国卫生研究院文献>PLoS Clinical Trials >Sudden Collapse of Vacuoles in Saintpaulia sp. Palisade Cells Induced by a Rapid Temperature Decrease
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Sudden Collapse of Vacuoles in Saintpaulia sp. Palisade Cells Induced by a Rapid Temperature Decrease

机译：刺胞突然崩溃在非洲堇sp。温度快速下降诱导的栅栏细胞

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摘要

It is well known that saintpaulia leaf is damaged by the rapid temperature decrease when cold water is irrigated onto the leaf surface. We investigated this temperature sensitivity and the mechanisms of leaf damage in saintpaulia (Saintpaulia sp. cv. ‘Iceberg’) and other Gesneriaceae plants. Saintpaulia leaves were damaged and discolored when subjected to a rapid decrease in temperature, but not when the temperature was decreased gradually. Sensitivity to rapid temperature decrease increased within 10 to 20 min during pre-incubation at higher temperature. Injury was restricted to the palisade mesophyll cells, where there was an obvious change in the color of the chloroplasts. During a rapid temperature decrease, chlorophyll fluorescence monitored by a pulse amplitude modulated fluorometer diminished and did not recover even after rewarming to the initial temperature. Isolated chloroplasts were not directly affected by the rapid temperature decrease. Intracellular pH was monitored with a pH-dependent fluorescent dye. In palisade mesophyll cells damaged by rapid temperature decrease, the cytosolic pH decreased and the vacuolar membrane collapsed soon after a temperature decrease. In isolated chloroplasts, chlorophyll fluorescence declined when the pH of the medium was lowered. These results suggest that a rapid temperature decrease directly or indirectly affects the vacuolar membrane, resulting in a pH change in the cytosol that subsequently affects the chloroplasts in palisade mesophyll cells. We further confirmed that the same physiological damage occurs in other Gesneriaceae plants. These results strongly suggested that the vacuoles of palisade mesophyll cells collapsed during the initial phase of leaf injury.
机译：众所周知，当将冷水灌溉到叶子表面时，非洲菊的叶子会因温度的快速下降而受损。我们调查了这种温度敏感性以及非洲菊（Saintpaulia sp。cv。“ Iceberg”）和其他Gesneriaceae植物的叶片损伤机制。当温度迅速降低时，非洲菊的叶子受损并变色，而温度逐渐降低时则没有。在较高温度下预温育过程中，温度快速下降的敏感性在10到20分钟内增加。损伤仅限于栅栏的叶肉细胞，其中叶绿体的颜色发生明显变化。在温度快速下降的过程中，通过脉冲幅度调制荧光计监测的叶绿素荧光减弱，即使恢复到初始温度也无法恢复。分离的叶绿体不受温度快速下降的直接影响。用pH依赖性荧光染料监测细胞内pH。在温度迅速下降而受损的栅栏叶肉细胞中，温度下降后，胞质pH下降，液泡膜塌陷。在分离的叶绿体中，当培养基的pH降低时，叶绿素荧光减弱。这些结果表明，温度的快速下降直接或间接地影响了液泡膜，导致了细胞质中的pH值改变，进而影响了栅栏叶肉细胞中的叶绿体。我们进一步证实，其他菊苣科植物也发生相同的生理损害。这些结果强烈表明，在叶损伤的初始阶段，栅栏叶肉细胞的液泡塌陷。

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