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首页> 美国卫生研究院文献>PLoS Clinical Trials >Aerobactin Synthesis Genes iucA and iucC Contribute to the Pathogenicity of Avian Pathogenic Escherichia coli O2 Strain E058
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Aerobactin Synthesis Genes iucA and iucC Contribute to the Pathogenicity of Avian Pathogenic Escherichia coli O2 Strain E058

机译：航空杆菌素合成基因iucA和iucC有助于禽致病性大肠杆菌O2株E058的致病性。

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Aerobactin genes are known to be present in virulent strains and absent from avirulent strains, but contributions of iucC and iucA, which are involved in aerobactin synthesis, to the pathogenicity of avian pathogenic Escherichia coli (APEC) have not been clarified. In this study, effects of double mutants (iucA/iutA or iucC/iutA) compared to those of single mutants (iucA, iucC or iutA) of aerobactin genes on the virulence of APEC strain E058 were examined both in vitro (aerobactin production, ingestion into HD-11 cells, survival in chicken serum) and in vivo (competitive growth against parental strain, colonization and persistence). In competitive co-infection assays, compared to the E058 parental strain, the E058ΔiucA mutant was significantly reduced in the liver, kidney, spleen (all P<0.01), heart and lung (both P<0.001). The E058ΔiutA mutant also was significantly reduced in the liver, lung, kidney (all P<0.01), heart and spleen (both P<0.001). The E058ΔiucC mutant was significantly attenuated in the heart and kidney (both P<0.05) and showed a remarkable reduction in the liver, spleen and lung (P<0.01); meanwhile, both E058ΔiucAΔiutA and E058ΔiucCΔiutA double mutants were sharply reduced as well (P<0.001). In colonization and persistence assays, compared with E058, recovered colonies of E058ΔiucA were significantly reduced from the lung, liver, spleen and kidney (P<0.01) and significantly reduced in the heart (P<0.001). E058ΔiutA was significantly reduced from the heart, lung, liver, spleen and kidney (P<0.01). E058ΔiucC, E058ΔiucAΔiutA and E058ΔiucCΔiutA were significantly decreased in all organs tested (P<0.001). These results suggest that iutA, iucA and iucC play important roles in the pathogenicity of APEC E058.
机译：已知航空杆菌素基因存在于强毒菌株中，而无毒力菌株不存在，但是尚未阐明涉及航空细菌素合成的iucC和iucA对禽病原性大肠杆菌（APEC）的致病性的贡献。在这项研究中，双重突变体（iucA / iutA或iucC / iutA）与单个突变体（iucA，iucC或iutA）的空气杆菌素基因对APEC E058毒力的影响均在体外进行了研究（空气杆菌素的产生，摄入）进入HD-11细胞，在鸡血清中存活）和体内（针对亲本菌株的竞争性生长，定植和持久性）。在竞争性共感染试验中，与E058亲本菌株相比，E058ΔiucA突变体在肝脏，肾脏，脾脏（所有P <0.01），心脏和肺（均P <0.001）中均显着降低。 E058ΔiutA突变体在肝，肺，肾（所有 P <0.01），心脏和脾脏（均 P <0.001）中也显着降低。 E058Δ iucC 突变体在心脏和肾脏中显着减毒（均 P <0.05），并在肝，脾和肺中显着降低（ P < /em><0.01）;同时，E058Δ iucA Δ iutA 和E058Δ iucC Δ iutA 双重突变体也急剧减少（ P <0.001）。在定植和持久性测定中，与E058相比，从肺，肝，脾和肾中回收的E058Δ iucA 菌落显着减少（ P <0.01），而E058Δ iucA 则显着减少。心脏（ P <0.001）。心脏，肺，肝，脾和肾中的E058Δ iutA 显着降低（ P <0.01）。 E058Δ iucC ，E058Δ iucA Δ iutA 和E058Δ iucC Δ iutA 在所有测试的器官中（ P <0.001）。这些结果表明 iutA ， iucA 和 iucC 在APEC E058的致病性中起重要作用。

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