• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>PLoS Clinical Trials >A Comprehensive Census of Microbial Diversity in Hot Springs of Tengchong, Yunnan Province China Using 16S rRNA Gene Pyrosequencing
【2h】

A Comprehensive Census of Microbial Diversity in Hot Springs of Tengchong, Yunnan Province China Using 16S rRNA Gene Pyrosequencing

机译：16S rRNA基因焦磷酸测序技术在云南腾冲温泉的微生物多样性综合调查

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

The Rehai and Ruidian geothermal fields, located in Tengchong County, Yunnan Province, China, host a variety of geochemically distinct hot springs. In this study, we report a comprehensive, cultivation-independent census of microbial communities in 37 samples collected from these geothermal fields, encompassing sites ranging in temperature from 55.1 to 93.6°C, in pH from 2.5 to 9.4, and in mineralogy from silicates in Rehai to carbonates in Ruidian. Richness was low in all samples, with 21–123 species-level OTUs detected. The bacterial phylum Aquificae or archaeal phylum Crenarchaeota were dominant in Rehai samples, yet the dominant taxa within those phyla depended on temperature, pH, and geochemistry. Rehai springs with low pH (2.5–2.6), high temperature (85.1–89.1°C), and high sulfur contents favored the crenarchaeal order Sulfolobales, whereas those with low pH (2.6–4.8) and cooler temperature (55.1–64.5°C) favored the Aquificae genus Hydrogenobaculum. Rehai springs with neutral-alkaline pH (7.2–9.4) and high temperature (>80°C) with high concentrations of silica and salt ions (Na, K, and Cl) favored the Aquificae genus Hydrogenobacter and crenarchaeal orders Desulfurococcales and Thermoproteales. Desulfurococcales and Thermoproteales became predominant in springs with pH much higher than the optimum and even the maximum pH known for these orders. Ruidian water samples harbored a single Aquificae genus Hydrogenobacter, whereas microbial communities in Ruidian sediment samples were more diverse at the phylum level and distinctly different from those in Rehai and Ruidian water samples, with a higher abundance of uncultivated lineages, close relatives of the ammonia-oxidizing archaeon “Candidatus Nitrosocaldus yellowstonii”, and candidate division O1aA90 and OP1. These differences between Ruidian sediments and Rehai samples were likely caused by temperature, pH, and sediment mineralogy. The results of this study significantly expand the current understanding of the microbiology in Tengchong hot springs and provide a basis for comparison with other geothermal systems around the world.
机译：位于中国云南省腾冲县的瑞海和瑞甸地热田拥有各种地球化学独特的温泉。在这项研究中，我们报告了从这些地热田收集的37个样品中微生物群落的全面，独立于种植的普查，这些样品的温度范围为55.1至93.6°C，pH为2.5至9.4，以及硅酸盐的硅酸盐矿物学瑞海到瑞甸的碳酸盐岩。所有样品的丰富度都很低，检测到21-123种物种水平的OTU。在Rehai样本中，细菌门属Aquificae或古细菌门Crenarchaeota占优势，但这些门中的优势类群取决于温度，pH和地球化学。 pH值低（2.5–2.6），高温（85.1–89.1°C）和高硫含量的瑞海泉水更适合于月牙鱼阶磺球菌，而pH值低（2.6–4.8）和温度较低（55.1–64.5°C） ）偏爱Aquificae属Hydrogenobaculum。具有中性碱性pH（7.2-9.4）和高温（> 80°C）且硅和盐离子浓度较高（Na，K和Cl）的Rehai泉水更喜欢Aquificae属Hydrogenobacter和crenarchaeal命令Desulfurococcales和Thermoproteales。在春季，脱硫球菌和热蛋白酶占主导地位，其pH值远高于这些订单已知的最佳pH值甚至最高pH值。瑞甸水样品中只有一个水产杆菌属属双歧杆菌，而瑞甸沉积物样品中的微生物群落在门类水平上更加多样，与瑞海和瑞甸水样品中的微生物群落明显不同，未耕种谱系含量较高，氨的近亲属氧化古细菌“ Candidatus Nitrosocaldus yellowstonii”，以及候选分类O1aA90和OP1。瑞甸沉积物和热海样品之间的这些差异可能是由温度，pH值和沉积物矿物学造成的。这项研究的结果极大地扩展了当前对腾冲温泉微生物学的理解，并为与世界其他地热系统进行比较提供了基础。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号