首页> 美国卫生研究院文献>PLoS Clinical Trials >Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti
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Glytube: A Conical Tube and Parafilm M-Based Method as a Simplified Device to Artificially Blood-Feed the Dengue Vector Mosquito, Aedes aegypti

机译:Glytube:一种基于锥形管和Parafilm M的方法,作为一种人工为登革热媒介蚊,埃及伊蚊提供血液的简化装置

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摘要

Aedes aegypti, the main vector of dengue virus, requires a blood meal to produce eggs. Although live animals are still the main blood source for laboratory colonies, many artificial feeders are available. These feeders are also the best method for experimental oral infection of Ae. aegypti with Dengue viruses. However, most of them are expensive or laborious to construct. Based on principle of Rutledge-type feeder, a conventional conical tube, glycerol and Parafilm-M were used to develop a simple in-house feeder device. The blood feeding efficiency of this apparatus was compared to a live blood source, mice, and no significant differences (p = 0.1189) were observed between artificial-fed (51.3% of engorgement) and mice-fed groups (40.6%). Thus, an easy to assemble and cost-effective artificial feeder, designated “Glytube” was developed in this report. This simple and efficient feeding device can be built with common laboratory materials for research on Ae. aegypti.
机译:埃及伊蚊是登革热病毒的主要载体,需要血粉才能产卵。尽管活体动物仍然是实验室菌落的主要血液来源,但仍有许多人工饲养器可用。这些喂食器也是实验性口服Ae感染的最佳方法。埃及感染登革热病毒。然而,它们中的大多数昂贵或建造费力。根据Rutledge型给料器的原理,使用常规的锥形管,甘油和Parafilm-M来开发简单的内部给料器设备。将这种装置的供血效率与活血源,小鼠进行了比较,在人工喂养(充血量的51.3%)和小鼠喂养组(40.6%)之间未观察到显着差异(p = 0.1189)。因此,在本报告中开发了一种易于组装且具有成本效益的人工进料器,称为“ Glytube”。这种简单而有效的饲喂装置可以用普通的实验室材料建造,用于Ae的研究。埃及。

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