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首页> 美国卫生研究院文献>PLoS Clinical Trials >Biological Control Activities of Rice-Associated Bacillus sp. Strains against Sheath Blight and Bacterial Panicle Blight of Rice
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Biological Control Activities of Rice-Associated Bacillus sp. Strains against Sheath Blight and Bacterial Panicle Blight of Rice

机译：水稻相关芽孢杆菌的生物防治活性。水稻对纹枯病和细菌性穗病的抗性

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Potential biological control agents for two major rice diseases, sheath blight and bacterial panicle blight, were isolated from rice plants in this study. Rice-associated bacteria (RABs) isolated from rice plants grown in the field were tested for their antagonistic activities against the rice pathogens, Rhizoctonia solani and Burkholderia glumae, which cause sheath blight and bacterial panicle blight, respectively. Twenty-nine RABs were initially screened based on their antagonistic activities against both R. solani and B. glumae. In follow-up retests, 26 RABs of the 29 RABs were confirmed to have antimicrobial activities, but the rest three RABs did not reproduce any observable antagonistic activity against R. solani or B. glumae. According to16S rDNA sequence identity, 12 of the 26 antagonistic RABs were closest to Bacillus amyloliquefaciens, while seven RABs were to B. methylotrophicus and B, subtilis, respectively. The 16S rDNA sequences of the three non-antagonistic RABs were closest to Lysinibacillus sphaericus (RAB1 and RAB12) and Lysinibacillus macroides (RAB5). The five selected RABs showing highest antimicrobial activities (RAB6, RAB9, RAB16, RAB17S, and RAB18) were closest to B. amyloliquefaciens in DNA sequence of 16S rDNA and gyrB, but to B. subtilis in that of recA. These RABs were observed to inhibit the sclerotial germination of R. solani on potato dextrose agar and the lesion development on detached rice leaves by artificial inoculation of R. solani. These antagonistic RABs also significantly suppressed the disease development of sheath blight and bacterial panicle blight in a field condition, suggesting that they can be potential biological control agents for these rice diseases. However, these antagonistic RABs showed diminished disease suppression activities in the repeated field trial conducted in the following year probably due to their reduced antagonistic activities to the pathogens during the long-term storage in -70C, suggesting that development of proper storage methods to maintain antagonistic activity is as crucial as identification of new biological control agents.
机译：在这项研究中，从水稻中分离出了两种主要的水稻疾病潜在的生物防治剂，鞘枯病和细菌性圆锥花序病。测试了从田间生长的水稻植物中分离出的水稻相关细菌（RAB）对水稻病原体solani solani和Burkholderia glumae的拮抗活性，它们分别引起鞘枯萎病和细菌性穗枯萎病。最初根据它们对茄形梭菌和布鲁氏芽孢杆菌的拮抗活性筛选了29个RAB。在后续的复查中，确认了29种RAB中的26种RAB具有抗菌活性，但其余3种RAB对sol。solani或B. glumae均未产生任何可观察到的拮抗活性。根据16S rDNA序列同一性，在26种拮抗性RAB中，有12种最接近解淀粉芽孢杆菌，而7种RAB分别是甲基芽孢杆菌和枯草芽孢杆菌。三种非拮抗RABs的16S rDNA序列最接近球形乳杆菌Lysinibacillus sphaericus（RAB1和RAB12）和大乳杆菌Lysinibacillus macroides （RAB5）。五个具有最高抗菌活性的RAB（RAB6，RAB9，RAB16，RAB17S和RAB18）最接近 B 。 16S rDNA和 gyrB 的DNA序列中的 amyloliquefaciens ，但 B 则是。 recA 中的 subtilis 。观察到这些RAB抑制了 R 的菌核萌发。人工接种 R 对马铃薯葡萄糖琼脂的 solani 和离体稻叶上的病变发展。 solani 。这些拮抗性RABs在田间条件下也显着抑制鞘枯病和细菌性穗枯病的病情发展，表明它们可能是这些水稻病害的潜在生物防治剂。但是，这些拮抗的RAB在次年的重复田间试验中显示出减弱的疾病抑制活性，这可能是由于它们在-70C的长期储存过程中对病原体的拮抗活性降低了，这表明开发了适当的储存方法来维持拮抗作用活性与鉴定新的生物防治剂一样重要。

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