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Phylogeography of the termite Macrotermes gilvus and insight into ancient dispersal corridors in Pleistocene Southeast Asia

机译:白蚁Macrotermes gilvus的系统记录和对更新世东南亚古代扩散走廊的认识

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摘要

Dispersal of soil-dwelling organisms via the repeatedly exposed Sunda shelf through much of the Pleistocene in Southeast Asia has not been studied extensively, especially for invertebrates. Here we investigated the phylogeography of an endemic termite species, Macrotermes gilvus (Hagen), to elucidate the spatiotemporal dynamics of dispersal routes of terrestrial fauna in Pleistocene Southeast Asia. We sampled 213 termite colonies from 66 localities throughout the region. Independently inherited microsatellites and mtDNA markers were used to infer the phylogeographic framework of M. gilvus. Discrete phylogeographic analysis and molecular dating based on fossil calibration were used to infer the dynamics of M. gilvus dispersal in time and space across Southeast Asia. We found that the termite dispersal events were consistently dated within the Pleistocene time frame. The dispersal pattern was multidirectional, radiating eastwards and southwards out of Indochina, which was identified as the origin for dispersal events. We found no direct dispersal events between Sumatra and Borneo despite the presence of a terrestrial connection between them during the Pleistocene. Instead, central Java served as an important link allowing termite colonies to be established in Borneo and Sumatra. Our findings support the hypothesis of a north-south dispersal corridor in Southeast Asia and suggest the presence of alternative dispersal routes across Sundaland during the Pleistocene. For the first time, we also propose that a west-east dispersal through over-water rafting likely occurred across the Pleistocene South China Sea. We found at least two independent entry routes for terrestrial species to infiltrate Sumatra and Borneo at different times.
机译:尚未广泛研究通过反复暴露的Sun他(Sunda)架子通过东南亚大部分更新世散布土壤生物的情况,尤其是对无脊椎动物。在这里,我们调查了特有白蚁物种Macrotermes gilvus(Hagen)的系统地理学,以阐明更新世东南亚陆生动物区系传播途径的时空动态。我们从该地区的66个地方采样了213个白蚁殖民地。使用独立遗传的微卫星和mtDNA标记来推断吉尔伯勒氏菌的系统地理框架。基于化石校准的离散系统地理分析和分子测年被用来推断整个东南亚的时间和空间上的吉尔曼分布。我们发现白蚁扩散事件始终在更新世时期内确定。散布模式是多向的,从印度支那向东和向南辐射,这被确定为散布事件的起源。尽管在更新世期间苏门答腊和婆罗洲之间没有地面联系,但我们没有发现它们之间有直接的传播事件。取而代之的是,中央Java作为重要的链接,允许在婆罗洲和苏门答腊建立白蚁殖民地。我们的发现支持了东南亚南北分散走廊的假说,并暗示了在更新世期间存在遍及Sun他兰的替代分散路线。我们还首次提出,在整个更新世南中国海可能通过水上漂流向西扩散。我们发现至少有两个独立的陆生物种进入途径,可在不同时间渗透到苏门答腊和婆罗洲。

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