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首页> 美国卫生研究院文献>PLoS Clinical Trials >Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense
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Metabolic profiling of two maize (Zea mays L.) inbred lines inoculated with the nitrogen fixing plant-interacting bacteria Herbaspirillum seropedicae and Azospirillum brasilense

机译：两种与固氮植物相互作用细菌草药草和巴西固氮菌接种的玉米（Zea mays L.）近交系的代谢谱分析

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Maize roots can be colonized by free-living atmospheric nitrogen (N2)-fixing bacteria (diazotrophs). However, the agronomic potential of non-symbiotic N2-fixation in such an economically important species as maize, has still not been fully exploited. A preliminary approach to improve our understanding of the mechanisms controlling the establishment of such N2-fixing associations has been developed, using two maize inbred lines exhibiting different physiological characteristics. The bacterial-plant interaction has been characterized by means of a metabolomic approach. Two established model strains of Nif+ diazotrophic bacteria, Herbaspirillum seropedicae and Azospirillum brasilense and their Nif- couterparts defficient in nitrogenase activity, were used to evaluate the impact of the bacterial inoculation and of N2 fixation on the root and leaf metabolic profiles. The two N2-fixing bacteria have been used to inoculate two genetically distant maize lines (FV252 and FV2), already characterized for their contrasting physiological properties. Using a well-controlled gnotobiotic experimental system that allows inoculation of maize plants with the two diazotrophs in a N-free medium, we demonstrated that both maize lines were efficiently colonized by the two bacterial species. We also showed that in the early stages of plant development, both bacterial strains were able to reduce acetylene, suggesting that they contain functional nitrogenase activity and are able to efficiently fix atmospheric N2 (Fix+). The metabolomic approach allowed the identification of metabolites in the two maize lines that were representative of the N2 fixing plant-bacterial interaction, these included mannitol and to a lesser extend trehalose and isocitrate. Whilst other metabolites such as asparagine, although only exhibiting a small increase in maize roots following bacterial infection, were specific for the two Fix+ bacterial strains, in comparison to their Fix- counterparts. Moreover, a number of metabolites exhibited a maize-genotype specific pattern of accumulation, suggesting that the highly diverse maize genetic resources could be further exploited in terms of beneficial plant-bacterial interactions for optimizing maize growth, with reduced N fertilization inputs.
机译：可以通过自由活动的固定大气氮（N2）的细菌（根瘤菌）来定居玉米根。然而，在诸如玉米等经济上重要的物种中，非共生N2固定的农艺潜力仍未得到充分开发。利用两种表现出不同生理特性的玉米自交系，已经开发出了一种初步的方法来改善我们对控制这种N2固定缔合机制的理解。细菌-植物相互作用已通过代谢组学方法进行了表征。使用两个已建立的Nif + 重氮营养细菌模型菌株，草拟螺旋藻和巴西拟螺螺旋藻，以及它们的Nif -库珀部件的固氮酶活性低，来评估细菌接种和感染的影响。 N2固定对根和叶代谢谱的影响。两种固定氮的细菌已被用于接种两个遗传距离远的玉米品系（FV252和FV2），这些玉米品系已经以其鲜明的生理特性为特征。使用一个可控的gnotobiotic实验系统，该系统允许在无氮培养基中用两种重氮菌接种玉米植物，我们证明了两种玉米都有效地被这两种细菌定殖。我们还表明，在植物发育的早期，两种细菌菌株均能够还原乙炔，这表明它们均具有功能性固氮酶活性，并且能够有效固定大气中的N2（Fix + ）。代谢组学方法允许鉴定两个玉米系中代表N2固定的植物-细菌相互作用的代谢产物，其中包括甘露醇和较小范围的海藻糖和异柠檬酸盐。尽管其他代谢物（例如天冬酰胺），尽管在细菌感染后仅在玉米根部表现出少量增加，但与两种Fix + 细菌菌株相比，它们的Fix -具有特异性同行。此外，许多代谢物表现出特定的玉米基因型积累模式，这表明可以通过有利的植物-细菌相互作用进一步利用高度多样化的玉米遗传资源来优化玉米生长，同时减少氮肥投入。

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