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Ancient DNA analysis of Indigenous rockfish use on the Pacific Coast: Implications for marine conservation areas and fisheries management

机译:太平洋沿岸土著石鱼使用的古代DNA分析:对海洋保护区和渔业管理的启示

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摘要

Rockfish (Sebastes spp.) are a common marine fish in nearshore and continental shelf environments in the North Pacific Ocean. They are frequently identified in coastal archaeological sites in western North America; however, the morphological similarity of rockfish species limits conventional zooarchaeological identifications to the genus level. This study applies ancient DNA analysis to 96 archaeological rockfish specimens from four sites on separate islands in an archipelago on western Vancouver Island, British Columbia, Canada. Two of the archaeological sites are located within a marine protected area specifically designed to facilitate the recovery of inshore rockfish populations; two sites are located outside this boundary and remain subject to considerable fishing pressure. Using mitochondrial 16S and control region DNA sequences, we identify at least twelve different rockfish species utilized during the past 2,500 years. Identification of rockfish at closely spaced and contemporaneously occupied sites confirms that a variety of Sebastes species were consistently exploited at each site, with more exposed areas having a higher number of species present. Identification results indicate that four of the twelve species did not occur within the conservation area boundary and, instead, were found in sites where commercial and recreational fishing continues to be permitted. This study demonstrates that ancient DNA identifications of archaeological assemblages can complement and expand perspective on modern day fisheries conservation and management in this National Park Reserve and First Nations ancestral territory.
机译:石鱼(Sebastes spp。)是北太平洋近岸和大陆架环境中的常见海水鱼。它们经常在北美西部的沿海考古现场被发现。然而,石鱼物种的形态相似性将常规的动物考古学鉴定限制在属水平。这项研究对来自加拿大不列颠哥伦比亚省温哥华岛西部一个群岛上四个岛屿上四个地点的96个考古石鱼标本进行了古代DNA分析。其中两个考古遗址位于专门为促进近海石鱼种群恢复而设计的海洋保护区内;有两个地点位于该边界以外,仍然承受相当大的捕鱼压力。使用线粒体16S和控制区DNA序列,我们可以确定在过去2500年中使用的至少十二种不同的石鱼物种。在近距离并同时被占领的地点鉴定出石鱼证实了在每个地点都一致开发了多种Sebastes物种,暴露的区域越多,物种的数量就越多。鉴定结果表明,十二种中的四种没有出现在保护区范围内,而是在继续允许商业和休闲捕鱼的地点发现。这项研究表明,考古学组合的古代DNA鉴定可以补充和扩展对这个国家公园保护区和原住民祖先地区现代渔业保护和管理的认识。

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