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首页> 美国卫生研究院文献>PLoS Clinical Trials >Environmental factors influence the Haloferaxvolcanii S-layer protein structure
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Environmental factors influence the Haloferaxvolcanii S-layer protein structure

机译：环境因素影响Haloferax火山S层蛋白结构

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摘要

S-layers commonly cover archaeal cell envelopes and are composed of proteins that self-assemble into a paracrystalline surface structure. Despite their detection in almost all archaea, there are few reports investigating the structural properties of these proteins, with no reports exploring this topic for halophilic S-layers. The objective of the present study was to investigate the secondary and tertiary organization of the Haloferax volcanii S-layer protein. Such investigations were performed using circular dichroism, fluorescence spectroscopy, dynamic light scattering and transmission electron microscopy. The protein secondary structure is centered on β-sheets and is affected by environmental pH, with higher disorder in more alkaline conditions. The pH can also affect the protein’s tertiary structure, with higher tryptophan side-chain exposure to the medium under the same conditions. The concentrations of Na, Mg and Ca ions in the environment also affect the protein structures, with small changes in α-helix and β-sheet content, as well as changes in tryptophan side chain exposure. These changes in turn influence the protein’s functional properties, with cell envelope preparations revealing striking differences when in different salt conditions. Thermal denaturation assaysrevealed that the protein is stable. It has been reported that the S-layerprotein N-glycosylation process is affected by external factorsand the present study indicates for the first time changes in the proteinstructure.
机译：S层通常覆盖古细菌细胞包膜，并由自组装成顺晶表面结构的蛋白质组成。尽管在几乎所有古细菌中都检​​测到了它们，但很少有报道研究这些蛋白质的结构特性，也没有报道探讨有关嗜盐S层的话题。本研究的目的是调查Haloferax volcanii S层蛋白的二级和三级组织。使用圆二色性，荧光光谱，动态光散射和透射电子显微镜进行了此类研究。蛋白质二级结构以β-折叠为中心，受环境pH值的影响，在更碱性的条件下具有更高的无序性。在相同条件下，pH值较高的色氨酸侧链也可影响蛋白质的三级结构。环境中Na，Mg和Ca离子的浓度也会影响蛋白质结构，α-螺旋和β-折叠含量以及色氨酸侧链暴露的变化很小。这些变化反过来会影响蛋白质的功能特性，在不同的盐条件下，细胞被膜的制备也显示出惊人的差异。热变性测定揭示蛋白质是稳定的。据报道，S层蛋白N-糖基化过程受外部因素影响本研究首次表明蛋白质的变化结构体。

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