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首页> 美国卫生研究院文献>PLoS Clinical Trials >Production of bioactive compounds with bactericidal and antioxidant potential by endophytic fungus Alternaria alternata AE1 isolated from Azadirachta indica A. Juss.
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Production of bioactive compounds with bactericidal and antioxidant potential by endophytic fungus Alternaria alternata AE1 isolated from Azadirachta indica A. Juss.

机译：分离自印度印za（Azadirachta indica A. Juss）的内生真菌Alternaria alternata AE1生产具有杀菌和抗氧化潜力的生物活性化合物。

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For combating multidrug-resistant microorganisms, exploration of natural compounds from plant endophytes increases the chance of finding novel compounds. An efficient bioactive metabolites producing endophytic fungal strain AE1 was isolated from leaves of Azadirachta indica A. Juss. The metabolites were found to be thermostable, non-proteinacious and produced prominent zones of inhibition against numbers of Gram positive and Gram negative bacteria. Based on 28S rDNA (D1/D2) sequence homology the isolate AE1 was identified as Alternaria alternata. Malt extract broth was found effective for the maximum production of bioactive metabolites by the isolate and was subjected for solvent extraction. The Ethyl acetate (EA) fraction of AE1 showed MIC values of 300–400 μg/ml against Gram positive and Gram negative bacteria tested. The cidal mode of action of EA fraction was detected by treating bacterial cultures at mid log phase. Scanning electron microscopic study supported morphological disintegration of bacterial cells. Release of nucleic acid, protein and potassium ions (K+) also suggested lysis of bacterial cells or leakage of cell membrane upon treatment. In addition, reduction of the activity of EMP pathway, TCA cycle and gluconeogenic enzymes in all bacteria suggested the interference of antibacterial principles with central carbohydrate metabolic pathways. Thin layer chromatographic separation followed by GC-MS analysis of EA fraction suggested numbers of antimicrobial compound production by AE1. In addition, DPPH free radical as well as superoxide radical scavenging assay also suggested strong antioxidant potential of AE1 with an IC50 value of 38.0±1.7 μg/ml and 11.38±1.2 μg/ml respectively. On the basis of above facts it can be concluded that the strain AE1 will be a good source of bioactive compounds having medicinal importance.
机译：为了对抗多药耐药微生物，从植物内生菌中探索天然化合物会增加发现新化合物的机会。从印za（Azadirachta indica A. Juss）的叶片中分离出产生内生真菌菌株AE1的有效生物活性代谢物。发现这些代谢物是热稳定的，非蛋白质的，并且产生了针对革兰氏阳性和革兰氏阴性细菌数量的显着抑制区域。基于28S rDNA（D1 / D2）序列同源性，分离株AE1被鉴定为Alternaria alternata。发现麦芽提取物肉汤对于分离物最大程度地产生生物活性代谢物有效，并用于溶剂提取。相对于所测试的革兰氏阳性和革兰氏阴性细菌，AE1的乙酸乙酯（EA）馏分的MIC值为300–400μg/ ml。通过在对数中期对细菌培养物进行处理，可以检测到EA组分的杀灭作用模式。扫描电子显微镜研究支持细菌细胞的形态分解。核酸，蛋白质和钾离子（K + ）的释放也提示细菌细胞溶解或处理后细胞膜渗漏。此外，所有细菌中EMP途径，TCA周期和糖异生酶的活性降低表明抗菌原理对中枢碳水化合物代谢途径的干扰。薄层色谱分离，然后对EA馏分进行GC-MS分析，表明AE1产生了抗菌化合物。此外，DPPH自由基和超氧化物自由基清除试验还表明AE1具有强大的抗氧化潜力，IC50值分别为38.0±1.7μg/ ml和11.38±1.2μg/ ml。基于上述事实，可以得出结论，菌株AE1将是具有医学重要性的生物活性化合物的良好来源。

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