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首页> 美国卫生研究院文献>Polymers >The Application of Multi-Walled Carbon Nanotubes in Bone Tissue Repair Hybrid Scaffolds and the Effect on Cell Growth In Vitro
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The Application of Multi-Walled Carbon Nanotubes in Bone Tissue Repair Hybrid Scaffolds and the Effect on Cell Growth In Vitro

机译：多孔碳纳米管在骨组织修复混合支架中的应用及对细胞体外生长的影响

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摘要

In this study, composite scaffolds with different multi-walled carbon nanotubes (MWCNTs) content were prepared by freeze-drying. These scaffolds were characterized by scanning electron microscope (SEM), energy dispersive spectroscopy (EDS), Fourier transform infrared spectroscopy (FTIR), porosity, hydrophilicity, mechanical strength, and degradation. The MWCNTs scaffolds were structurally sound and had porous structures that offered ample space for adherence, proliferation, and differentiation of MC3T3-E1 cells, and also supported the transport of nutrients and metabolic waste. CS/Gel/nHAp/0.3%MWCNTs scaffolds provided the best outcomes in terms of scaffold porosity, hydrophilicity, and degradation rate. However, CS/Gel/nHAp/0.6%MWCNTs scaffolds were found to support the optimal growth, homogenous distribution, and biological activity of MC3T3-E1 cells. The excellent properties of CS/Gel/nHAp/0.6%MWCNTs scaffolds for the adhesion, proliferation, and osteogenesis differentiation of MC3T3-E1 cells in vitro highlights the potential applications of this scaffold in bone tissue regeneration.
机译：在这项研究中，通过冷冻干燥制备了具有不同多壁碳纳米管（MWCNTs）含量的复合支架。这些支架通过扫描电子显微镜（SEM），能量色散光谱（EDS），傅立叶变换红外光谱（FTIR），孔隙率，亲水性，机械强度和降解来表征。 MWCNTs支架结构合理，具有多孔结构，为MC3T3-E1细胞的粘附，增殖和分化提供了充足的空间，还支持营养物质和代谢废物的运输​​。 CS / Gel / nHAp / 0.3％MWCNTs支架在支架孔隙率，亲水性和降解率方面提供了最佳结果。然而，发现CS / Gel / nHAp / 0.6％MWCNTs支架支持MC3T3-E1细胞的最佳生长，均匀分布和生物活性。 CS / Gel / nHAp / 0.6％MWCNTs支架在体外对MC3T3-E1细胞的粘附，增殖和成骨分化的优异性能突出了该支架在骨组织再生中的潜在应用。

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