• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Society for Endocrinology Open Access >Effects of ACTH dexamethasone and adrenalectomy on 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) gene expression in the rat central nervous system
【2h】

Effects of ACTH dexamethasone and adrenalectomy on 11β-hydroxylase (CYP11B1) and aldosterone synthase (CYP11B2) gene expression in the rat central nervous system

机译：ACTH地塞米松和肾上腺切除术对大鼠中枢神经系统11β-羟化酶（CYP11B1）和醛固酮合酶（CYP11B2）基因表达的影响

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

Using a highly sensitive quantitative RT-PCR method for the measurement of CYP11B1 (11β-hydroxylase) and CYP11B2 (aldosterone synthase) mRNAs, we previously demonstrated that CYP11B2 expression in the central nervous system (CNS) is subject to regulation by dietary sodium. We have now quantified the expression of these genes in the CNS of male Wistar Kyoto (WKY) rats in response to systemic ACTH infusion, dexamethasone infusion, and to adrenalectomy. CYP11B1 and CYP11B2 mRNA levels were measured in total RNA isolated from the adrenal gland and discrete brain regions using real-time quantitative RT-PCR. ACTH infusion (40 ng/day for 7 days, N=8) significantly increased CYP11B1 mRNA in the adrenal gland, hypothalamus, and cerebral cortex compared with animals infused with vehicle only. ACTH infusion decreased adrenal CYP11B2 expression but increased expression in all of the CNS regions except the cortex. Dexamethasone (10 μg/day for 7 days, N=8) reduced adrenal CYP11B1 mRNA compared with control animals but had no significant effect on either gene's expression in the CNS. Adrenalectomy (N=6 per group) significantly increased CYP11B1 expression in the hippocampus and hypothalamus and raised CYP11B2 expression in the cerebellum relative to sham-operated animals. This study confirms the transcription of CYP11B1 and CYP11B2 throughout the CNS and demonstrates that gene transcription is subject to differential regulation by ACTH and circulating corticosteroid levels.
机译：使用高灵敏的定量RT-PCR方法测量CYP11B1（11β-羟化酶）和CYP11B2（醛固酮合酶）mRNA的表达，我们先前证明CYP11B2在中枢神经系统（CNS）中的表达受饮食钠的调节。现在，我们已经对全身性ACTH输注，地塞米松输注和肾上腺切除术的反应对雄性Wistar Kyoto（WKY）大鼠中枢神经系统中这些基因的表达进行了定量。 CYP11B1和CYP11B2 mRNA水平使用实时定量RT-PCR测定从肾上腺和离散脑区分离的总RNA中。与仅注射赋形剂的动物相比，ACTH注射（40μng/天，连续7天，N = 8）显着增加了肾上腺，下丘脑和大脑皮层中CYP11B1 mRNA的表达。 ACTH输注可降低肾上腺CYP11B2的表达，但在除皮质外的所有CNS区域中均会增加表达。地塞米松（10μg/天，连续7天，N = 8）与对照组相比降低了肾上腺CYP11B1 mRNA的表达，但对中枢神经系统中任一基因的表达均无显着影响。与假手术动物相比，肾上腺切除术（每组N = 6）显着增加海马和下丘脑中CYP11B1的表达，并在小脑中提高CYP11B2的表达。这项研究证实了整个CNS中CYP11B1和CYP11B2的转录，并证明基因转录受ACTH和循环皮质类固醇水平的差异调节。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号