• 主题
高级搜索  >
历史搜索 清空历史
首页> 美国卫生研究院文献>Springer Open Choice >Comparison of rapid BACpro® II Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis
【2h】

Comparison of rapid BACpro® II Sepsityper® kit and in-house preparation methods for direct identification of bacteria from blood cultures by MALDI-TOF MS with and without Sepsityper® module analysis

机译：快速BACpro®IISepsityper®试剂盒与内部制备方法的比较该方法可通过MALDI-TOF MS在有和没有Sepsityper®模块分析的情况下直接鉴定血液培养物中的细菌

代理获取
本网站仅为用户提供外文OA文献查询和代理获取服务，本网站没有原文。下单后我们将采用程序或人工为您竭诚获取高质量的原文，但由于OA文献来源多样且变更频繁，仍可能出现获取不到、文献不完整或与标题不符等情况，如果获取不到我们将提供退款服务。请知悉。
页面导航
  • 摘要
  • 著录项
  • 相似文献
  • 相关主题

摘要

There are several approaches available for purifying microorganisms prior to matrix-assisted laser desorption ionization–time of flight mass spectrometry (MALDI-TOF MS) analysis. In the present study, rapid BACpro® II (Nittobo Medical Co., Ltd., Tokyo, Japan), a new application, has been compared with Sepsityper® kit (Bruker Daltonics, Billerica, USA) and an in-house method. Samples were also tested with two modules, standard and Sepsityper®, identified in the Bruker MALDI-TOF MS. The bottles having monomicrobial growth were included in the study according to Gram staining results. In total, two hundred blood culture bottles were included but there was no growth in one of the subcultures so 199 blood culture bottles were studied prospectively. With the standard MALDI-TOF MS analysis, rapid BACpro® II could successfully identify microorganisms in 174/199 (87.4%) of the bottles where Sepsityper® kit and in-house method were successful in 136/199 (68.3%) and 114/199 (57.3%), respectively. When the MALDI-TOF MS data were analysed by Sepsityper® module, the identification rates were increased to 94.4%, 82.1% and 69.8% (p < 0.001), respectively. In the Sepsityper® module, 72/73 (98.6%) of Gram-negative and 97/106 (91.5%) of Gram-positive microorganisms were detected by rapid BACpro® II method. The present study shows that rapid BACpro® II is a reliable preparation procedure and has higher rates of identification compared with Sepsityper® kit and in-house method. The use of the Sepsityper® module in blood cultures increases the chance of identification for all three methods studied.
机译：在基质辅助激光解吸电离-飞行时间质谱（MALDI-TOF MS）分析之前，有几种纯化微生物的方法。在本研究中，快速的BACpro®II（日本东京Nittobo Medical Co.，Ltd。）已与Sepsityper®试剂盒（美国Billerica的Bruker Daltonics）和内部方法进行了比较。样品还使用在Bruker MALDI-TOF MS中鉴定的两个模块（标准模块和Sepsityper®）进行了测试。根据革兰氏染色结果，具有单微生物生长的瓶子也包括在研究中。总共包括了200个血液培养瓶，但是其中一种亚培养液却没有生长，因此对199个血液培养瓶进行了前瞻性研究。通过标准的MALDI-TOF MS分析，快速的BACpro®II可以成功识别174/199（87.4％）个瓶子中的微生物，而Sepsityper®试剂盒和内部方法在136/199（68.3％）和114 /分别为199（57.3％）。当使用Sepsityper®模块分析MALDI-TOF MS数据时，识别率分别提高到94.4％，82.1％和69.8％（p <0.001）。在模块中，通过快速BACpro®II方法检测到72/73（98.6％）革兰氏阴性菌和97/106（91.5％）革兰氏阳性菌。本研究表明，与Sepsityper®试剂盒和内部方法相比，快速BACpro®II是可靠的制备程序，并且具有较高的鉴定率。在血液培养中使用Sepsityper®模块增加了对所有三种研究方法进行鉴定的机会。

著录项

相似文献

  • 外文文献
  • 中文文献
  • 专利
代理获取

客服邮箱：kefu@zhangqiaokeyan.com

京公网安备：11010802029741号 ICP备案号：京ICP备15016152号-6 六维联合信息科技 (北京) 有限公司©版权所有

  • 客服微信

  • 服务号