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Cytotoxic and antimicrobial effects of biosynthesized ZnO nanoparticles using of Chelidonium majus extract

机译:白屈菜提取物生物合成ZnO纳米粒子的细胞毒性和抗菌作用

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摘要

The basic goal of this study was to synthesize zinc oxide nanoparticles using the Chelidonium majus extract and asses their cytotoxic and antimicrobial properties. The synthesized ZnO NPs were characterized by UV-Vis, Scanning Electron Microscopy (SEM) with EDS profile, Fourier Transform Infrared Spectroscopy (FTIR), X-ray diffraction (XRD), Transmission Electron Microscopy (TEM) and Atomic Force Microscopy (AFM). The aforementioned methods confirmed that the size of synthesized ZnO nanoparticles was at the range of 10 nm. The antimicrobial activity of ZnO nanoparticles synthesized using the Ch. majus extract was tested against standard strains of bacteria (Staphylococcus aureus NCTC 4163, Pseudomonas aeruginosa NCTC 6749, Escherichia coli ATCC 25922), yeast (Candida albicans ATCC 10231), filamentous fungi (molds: Aspergillus niger ATCC 16404, dermatophytes: Trichophyton rubrum ATCC 28188), clinical strains of bacteria (Escherichia coli, Pseudomonas aeruginosa, Staphylococcus aureus) and yeast (Candida albicans). The study showed that zinc oxide nanoparticles were excellent antimicrobial agents. What is more, biologically synthesized ZnO nanoparticles demonstrate high efficiency in treatment of human non-small cell lung cancer A549.
机译:这项研究的基本目标是使用白屈菜(Chelidonium majus)提取物合成氧化锌纳米粒子,并评估其细胞毒性和抗菌特性。合成的ZnO NPs的特征在于紫外可见光,具有EDS轮廓的扫描电子显微镜(SEM),傅里叶变换红外光谱(FTIR),X射线衍射(XRD),透射电子显微镜(TEM)和原子力显微镜(AFM) 。前述方法证实了合成的ZnO纳米粒子的尺寸在10nm的范围内。用Ch合成ZnO纳米颗粒的抗菌活性。测试了majus提取物对标准菌株(金黄色葡萄球菌NCTC 4163,铜绿假单胞菌NCTC 6749,大肠杆菌ATCC 25922),酵母菌(白色念珠菌ATCC 10231),丝状真菌(霉菌:黑曲霉ATCC 16404,皮肤癣菌:Trichophyton)的标准菌株),细菌(大肠杆菌,铜绿假单胞菌,金黄色葡萄球菌)和酵母(白色念珠菌)的临床菌株。研究表明,氧化锌纳米颗粒是优异的抗菌剂。此外,生物合成的ZnO纳米颗粒在治疗人类非小细胞肺癌A549中显示出高效率。

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