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首页> 美国卫生研究院文献>Springer Open Choice >Comprehensive gene expression analysis of the NAC gene family under normal growth conditions hormone treatment and drought stress conditions in rice using near-isogenic lines (NILs) generated from crossing Aday Selection (drought tolerant) and IR64
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Comprehensive gene expression analysis of the NAC gene family under normal growth conditions hormone treatment and drought stress conditions in rice using near-isogenic lines (NILs) generated from crossing Aday Selection (drought tolerant) and IR64

机译：利用杂交后代（耐旱）和IR64产生的近等基因系（NIL）对水稻正常生长条件激素处理和干旱胁迫条件下NAC基因家族的全面基因表达分析

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The NAC (NAM, ATAF1/2 and CUC2) genes are plant-specific transcriptional factors known to play diverse roles in various plant developmental processes. We describe the rice (Oryza sativa) OsNAC genes expression profiles (GEPs) under normal and water-deficit treatments (WDTs). The GEPs of the OsNAC genes were analyzed in 25 tissues covering the entire life cycle of Minghui 63. High expression levels of 17 genes were demonstrated in certain tissues under normal conditions suggesting that these genes may play important roles in specific organs. We determined that 16 genes were differentially expressed under at least 1 phytohormone (NAA, GA3, KT, SA, ABA, and JA) treatment. To investigate the GEPs in the root, leaf, and panicle of three rice genotypes [e.g., 2 near-isogenic lines (NILs) and IR64], we used two NILs from a common genetic combination backcross developed by Aday Selection and IR64. WDTs were applied using the fraction of transpirable soil water at severe, mild, and control conditions. Transcriptomic analysis using a 44K oligoarray from Agilent was performed on all the tissue samples. We identified common and specific genes in all tissues from the two NILs under both WDTs, and the majority of the OsNAC genes that were activated were in the drought-tolerant IR77298-14-1-2-B-10 line compared with the drought-susceptible IR77298-14-1-2-B-13 or IR64. In IR77298-14-1-2-B-10, seventeen genes were very specific in their expression levels. Approximately 70 % of the genes from subgroups SNAC and NAM/CUC3 were activated in the leaf, but 37 % genes from subgroup SND were inactivated in the root compared with the control under severe stress conditions. These results provide a useful reference for the cloning of candidate genes from the specific subgroup for further functional analysis.Electronic supplementary materialThe online version of this article (doi:10.1007/s00438-012-0686-8) contains supplementary material, which is available to authorized users.
机译：NAC（NAM，ATAF1 / 2和CUC2）基因是植物特异性的转录因子，已知在各种植物发育过程中起着不同的作用。我们描述了在正常和缺水处理（WDT）下水稻（Oryza sativa）的OsNAC基因表达谱（GEP）。在覆盖明辉63整个生命周期的25个组织中分析了OsNAC基因的GEP。在正常条件下在某些组织中证明了17个基因的高表达水平，表明这些基因可能在特定器官中发挥重要作用。我们确定在至少一种植物激素（NAA，GA3，KT，SA，ABA和JA）处理下16个基因差异表达。为了研究三种水稻基因型[例如2个近等基因系（NIL）和IR64]的根，叶和穗中的GEP，我们使用了由Aday Selection和IR64开发的常见遗传组合回交中的两个NIL。在重度，温和和控制条件下，使用可蒸发土壤水分的一部分来施用WDT。使用来自安捷伦的44K寡阵列对所有组织样品进行转录组分析。我们在两个WDT下都从两个NILs的所有组织中鉴定出共同的和特定的基因，并且大多数被激活的OsNAC基因都在耐旱的IR77298-14-1-2-B-10系中，而干旱- IR77298-14-1-2-B-13或IR64。在IR77298-14-1-2-B-10中，有17个基因的表达水平非常高。与重度胁迫条件下的对照相比，SNAC和NAM / CUC3亚组的基因中约有70％在叶中被激活，但SND亚组的37％基因在根中被灭活了。这些结果为从特定亚组克隆候选基因以进行进一步的功能分析提供了有用的参考。电子补充材料本文的在线版本（doi：10.1007 / s00438-012-0686-8）包含补充材料，可用于授权用户。

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