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首页> 美国卫生研究院文献>Springer Open Choice >Development and Validation of an Anodic Stripping Voltammetric Method for Determination of Zn2+ Ions in Brain Microdialysate Samples
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Development and Validation of an Anodic Stripping Voltammetric Method for Determination of Zn2+ Ions in Brain Microdialysate Samples

机译：阳极溶出伏安法测定脑微透析液样品中Zn2 +离子的方法开发与验证

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摘要

An easy, rapid, and sensitive anodic stripping voltammetric method with a controlled growth mercury drop electrode has been developed and validated for the determination of Zn2+ ions in brain microdialysate samples obtained from rats. The considered level of the zinc concentration in the dialysate was 0.5–6 ppb. In the investigated method, the stripping step was carried out by using a differential pulse potential-time voltammetric excitation signal. The optimal experimental conditions as well as the instrumental and accumulation parameters and supporting electrolyte composition were investigated. The optimized method was validated for precision, linearity, and accuracy. Mean recovery 82–110% was achieved, the precision expressed by CV not greater than 7.6% and the linearity given by correlation coefficient not lower than 0.9988. The limit of detection was 0.1 ppb. No interferences were observed. Due to high linearity, precision, and sensitivity, the developed method may be successfully applied in the determination of zinc ions in microdialysate brain samples. The results obtained for the first time demonstrate detailed characteristics of the determination of zinc in the brain microdialysate fluid by the ASV method. It may be applied in a wide range of physiological and pharmacological studies which focus on very low zinc concentration/alteration in various compartments of the organisms.
机译：建立了一种可控的生长汞滴电极的简便，快速，灵敏的阳极溶出伏安法，并用于测定大鼠脑微透析液样品中Zn 2 + 离子的有效性。认为透析液中锌的浓度为0.5–6 ppb。在研究的方法中，剥离步骤是通过使用差分脉冲电位-时间伏安激发信号进行的。研究了最佳实验条件以及仪器和累积参数以及支持电解质的组成。对优化方法进行了精度，线性和准确性验证。平均回收率达到82-110％，CV表示的精度不大于7.6％，相关系数给出的线性不小于0.9988。检测限为0.1 ppb。没有观察到干扰。由于高线性度，精密度和灵敏性，该开发的方法可以成功地用于微量透析液脑样品中锌离子的测定。首次获得的结果证明了通过ASV方法测定脑微透析液中锌的详细特征。它可用于广泛的生理和药理研究中，这些研究的重点是在生物的各个区室中极低的锌浓度/改变。

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