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Microwave-Accelerated and Metal-Enhanced Fluorescence Myoglobin Detection on Silvered Surfaces: Potential Application to Myocardial Infarction Diagnosis

机译:镀银表面的微波加速和金属增强荧光肌红蛋白检测:在心肌梗死诊断中的潜在应用

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摘要

In this short paper, we describe a novel approach to both significantly accelerate and optically amplify fluorescence-based immunoassays. Our approach utilizes metal-enhanced fluorescence (MEF) to intrinsically optically amplify fluorescence signatures, which, when combined with the use of low-power microwaves to kinetically accelerate assays, provides for both ultrafast and ultrabright immunoassays. Surprisingly, the use of low-power microwaves and silver nanostructures provides for localized heating, concentrating the effect to the particles themselves as compared to the generic heating of the high dielectric assay fluid. We have subsequently applied our microwave-accelerated MEF approach to the detection of myoglobin, where its rapid quantification is paramount for the clinical assessment of an acute myocardial infarction.
机译:在这篇短文中,我们描述了一种新颖的方法来显着加速和光学放大基于荧光的免疫测定。我们的方法利用金属增强的荧光(MEF)固有地光学放大荧光特征,当结合使用低功率微波来动力学加速测定时,可提供超快和超亮免疫测定。出乎意料的是,与高介电质分析液的一般加热相比,低功率微波和银纳米结构的使用提供了局部加热,从而将影响集中于颗粒本身。我们随后将我们的微波加速MEF方法应用于肌红蛋白的检测,其中快速定量对于急性心肌梗死的临床评估至关重要。

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