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首页> 美国卫生研究院文献>Toxins >Biosorption of B-aflatoxins Using Biomasses Obtained from Formosa Firethorn [Pyracantha koidzumii (Hayata) Rehder]
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Biosorption of B-aflatoxins Using Biomasses Obtained from Formosa Firethorn [Pyracantha koidzumii (Hayata) Rehder]

机译：利用从福尔摩沙火棘[火棘（Hayata）Rehder]获得的生物质对B-黄曲霉毒素进行生物吸附。

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Mycotoxin adsorption onto biomaterials is considered as a promising alternative for decontamination without harmful chemicals. In this research, the adsorption of B-aflatoxins (AFB1 and AFB2) using Pyracantha koidzumii biomasses (leaves, berries and the mixture of leaves/berries) from aqueous solutions was explored. The biosorbent was used at 0.5% (w/v) in samples spiked with 100 ng/mL of B-aflatoxin standards and incubated at 40 °C for up to 24 h. A standard biosorption methodology was employed and aflatoxins were quantified by an immunoaffinity column and UPLC methodologies. The biosorbent-aflatoxin interaction mechanism was investigated from a combination of zeta potential (ζ), Fourier transform infrared spectroscopy (FTIR) and scanning electron microscopy (SEM). The highest aflatoxin uptakes were 86% and 82% at 6 h using leaves and the mixture of leaves/berries biomasses, respectively. A moderate biosorption of 46% was attained when using berries biomass. From kinetic studies, the biosorption process is described using the first order adsorption model. Evidence from FTIR spectra suggests the participation of hydroxyl, amine, carboxyl, amide, phosphate and ketone groups in the biosorption and the mechanism was proposed to be dominated by the electrostatic interaction between the negatively charged functional groups and the positively charged aflatoxin molecules. Biosorption by P. koidzumii biomasses has been demonstrated to be an alternative to conventional systems for B-aflatoxins removal.
机译：霉菌毒素在生物材料上的吸附被认为是一种无污染，无有害化学物质的有前途的替代方法。在这项研究中，探索了利用火棘生物量（叶，浆果和叶/浆果的混合物）从水溶液中吸附B-黄曲霉毒素（AFB1和AFB2）的方法。在掺有100 ng / mL B-黄曲霉毒素标准品的样品中以0.5％（w / v）的浓度使用生物吸附剂，并在40°C下孵育长达24小时。采用标准的生物吸附方法，并通过免疫亲和柱和UPLC方法对黄曲霉毒素进行定量。从ζ电势（ζ），傅里叶变换红外光谱（FTIR）和扫描电子显微镜（SEM）的组合研究了生物吸附剂-黄曲霉毒素的相互作用机理。使用叶片和叶片/浆果生物量的混合物时，在6 h时，最高的黄曲霉毒素摄入量分别为86％和82％。使用浆果生物质时，可实现46％的中等生物吸附。通过动力学研究，使用一级吸附模型描述了生物吸附过程。 FTIR光谱的证据表明，羟基，胺基，羧基，酰胺基，磷酸基和酮基参与了生物吸附，并且该机理被认为是由带负电的官能团与带正电的黄曲霉毒素分子之间的静电相互作用所决定的。业已证明，假单胞菌生物量的生物吸附作用是常规系统去除B-黄曲霉毒素的替代方法。

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