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Immobilization of Lysozyme on Cotton Fabrics: Synthesis, Characterization, and Activity

机译:溶菌酶在棉织物上的固定:合成,表征和活性。

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摘要

Lysozyme was attached to three cotton fabrics through a glycine amino acid linker esterified to cotton cellulose. Lysozyme bound to cotton twill using a carbodiimide-mediated coupling reaction gave the best lysozyme incorporation (16.1 mg/g of cotton). Cotton spunlace nonwoven fabric bound slightly less lysozyme (12.8 mg/g of cotton), but had the best antimicrobial activity. Woven print cloth bound the least lysozyme (4 mg/g of cotton). Characterization of lysozyme on the fabric was performed using Fourier transform infrared and nitrogen analysis. Increases in amide I and amide II infrared frequencies were evident with increased lysozyme incorporation. Lysozyme-cellulose conjugate activity was assessed with a turbidity clearing assay using Micrococcus lysodeikticus. The order of lysozyme-fabric antimicrobial activity was nonwoven > twill > print cloth.
机译:溶菌酶通过被酯化为棉纤维素的甘氨酸氨基酸接头连接到三种棉织物上。使用碳二亚胺介导的偶联反应将溶菌酶结合到棉质斜纹布上,可得到最佳的溶菌酶掺入(16.1 mg / g棉)。水刺棉无纺布结合的溶菌酶略少(12.8 mg / g棉),但具有最佳的抗菌活性。机织的印花布结合最少的溶菌酶(4毫克/克棉)。使用傅立叶变换红外和氮气分析对织物上的溶菌酶进行表征。随着溶菌酶掺入的增加,酰胺I和酰胺II红外频率明显增加。溶菌酶-纤维素结合物活性通过使用溶菌微球菌的浊度清除测定法进行评估。溶菌酶织物抗菌活性的顺序为:无纺布>斜纹布>印花布。

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