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首页> 外文期刊>Acta Biochimica et Biophysica Sinica >Novel cotton homeobox gene and its expression profiling in root development and in response to stresses and phytohormones
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Novel cotton homeobox gene and its expression profiling in root development and in response to stresses and phytohormones

机译：棉花同源异型盒新基因及其在根系发育以及对逆境和植物激素反应中的表达谱

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摘要

Homeodomain-leucine zipper (HD-Zip) proteins are transcriptional factors involved in plant development. In this study, one cDNA clone (Gossypium hirsutum homeobox1, designated GhHB1) encoding HD-Zip protein was isolated from a cotton root cDNA library. The GhHB1 cDNA is 1132 bp in length, including an 828 bp open reading frame that encodes a peptide with 275 amino acids, and 5′-/3′-untranslated regions. The predicted GhHB1 protein containing a homeodomain and a leucine-rich zipper motif shares relatively high identity with other plant HD-Zip proteins. Analysis using quantitative real-time RT-PCR indicated that the GhHB1 gene is predominantly expressed in roots and hypocotyls. Furthermore, GhHB1 transcripts were largely accumulated in early root development, and significantly reduced to very low levels as roots further developed, suggesting that the gene might function in the early development of roots. Under treatment with 1% NaCl, the expression level of the GhHB1 gene was dramatically increased in roots. Likewise, GhHB1 activity in roots was up-regulated by abscisic acid. These results imply that GhHB1 might play an important role in response to salt stress and to abscisic acid signaling.
机译：同源域亮氨酸拉链（HD-Zip）蛋白是植物发育中涉及的转录因子。在这项研究中，一个编码HD-Zip蛋白的cDNA克隆（陆地棉Homeobox1，命名为GhHB1）从棉花根cDNA文库中分离出来。 GhHB1 cDNA的长度为1132 bp，包括一个828 bp的开放阅读框，其编码具有275个氨基酸和5'-/ 3'-非翻译区的肽。包含同源域和富含亮氨酸的拉链基序的预测GhHB1蛋白与其他植物HD-Zip蛋白具有相对较高的同一性。使用定量实时RT-PCR的分析表明，GhHB1基因主要在根和下胚轴中表达。此外，GhHB1转录物在根的早期发育中大量积累，并且随着根的进一步发育而显着降低至非常低的水平，这表明该基因可能在根的早期发育中起作用。用1％NaCl处理后，根中GhHB1基因的表达水平显着增加。同样，脱落酸可上调根中的GhHB1活性。这些结果暗示GhHB1可能在响应盐胁迫和脱落酸信号传导中起重要作用。

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