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首页> 外文期刊>Acta Pharmacologica Sinica >Chemopreventive effect of dimethyl dicarboxylate biphenyl on malignant transformation of WB-F344 rat liver epithelial cells
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Chemopreventive effect of dimethyl dicarboxylate biphenyl on malignant transformation of WB-F344 rat liver epithelial cells

机译：二羧酸二甲酯联苯对WB-F344大鼠肝上皮细胞恶性转化的化学预防作用

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摘要

Aim: To study the potential chemopreventive effect of dimethyl dicarboxylate biphenyl (DDB), an anti-hepatitis drug, on hepatocarcinogenesis in vitro. Methods: The anti-carcinogenesis effect of DDB was assessed on a two-stage chemical oncogenesis model induced by 3-methylcholanthrene and 12-0-tetradecanoyl phorbol 13-acetate (TPA) with WB-F344 rat liver epithelial cells (WB-F344 cells) in vitro. A soft-agar colony formation assay was used to determine the tumorigenic potential of the transformed WB-F344 cells. The gap junc-tional intercellular communication (GJIC) was detected using the scrape loading/ dye transfer technique. Results: DDB at 1 umol/L, 2 umol/L, and 4 umol/L significantly prevented the malignant transformation of WB-F344 cells induced by 3-methylcholanthrene and TPA. The average number of transformed foci decreased dramatically by 10.0%, 37.2%, and 47.4%, respectively. In soft agar, a remarkable decrease in colony numbers was observed in transformed cells treated with 2 umol/L and 4 umol/L DDB. DDB at 1 umol/L, 2 umol/L, and 4 umol/L inhibited the downregulation of GJIC induced by TPA in a dose-dependent manner. The GJIC recovered to 25.6%, 34.6%, and 44.9%, respectively, of the control WB-F344 cells by DDB. Conclusion: DDB has a potential chemopreventive effect on hepatocarcinogenesis induced by carcinogens in vitro.
机译：目的：研究抗肝炎药物二羧酸二甲酯联苯（DDB）对肝癌的潜在化学预防作用。方法：用WB-F344大鼠肝上皮细胞（WB-F344细胞），在3-甲基胆碱和12-0-十四烷酰佛波醇13-乙酸盐（TPA）诱导的两阶段化学致癌模型中评估了DDB的抗癌作用。 ） 体外。使用软琼脂集落形成测定法来确定转化的WB-F344细胞的致瘤潜力。使用刮擦加载/染料转移技术检测间隙连接细胞间通讯（GJIC）。结果：1 umol / L，2 umol / L和4 umol / L的DDB显着阻止了3-甲基胆碱和TPA诱导的WB-F344细胞恶变。转化病灶的平均数量分别急剧下降了10.0％，37.2％和47.4％。在软琼脂中，在用2 umol / L和4 umol / L DDB处理的转化细胞中观察到集落数明显减少。 1 umol / L，2 umol / L和4 umol / L的DDB以剂量依赖性方式抑制TPA诱导的GJIC的下调。通过DDB，GJIC分别恢复到对照WB-F344细胞的25.6％，34.6％和44.9％。结论：DDB对体外致癌物诱导的肝癌发生具有潜在的化学预防作用。

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