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首页> 外文期刊>Acta Pharmacologica Sinica >β-Adrenoceptors potentiate α_1-adrenoceptor-mediated inotropic response in rat left atria
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β-Adrenoceptors potentiate α_1-adrenoceptor-mediated inotropic response in rat left atria

机译：β-肾上腺素能受体增强大鼠左心房α_1-肾上腺素能受体介导的肌力反应

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AIM: To investigate whether stimulation of β-adrenoceptor (AR) and its subtypes augment α_1-AR-evoked positive inotropic response and inositol phosphate (InsP) accumulation in isolated rat left atria. METHODS: Inotropic response was determined by contractile function experiment in isolated electrically driven rat left atria. ~3H-InsP accumulations were measured by ~3H-inositol incorporation and column chromatography. RESULTS: (1) Stimulation of α_1-AR by phenylephrine (PE) or norepinephrine (NE) in the presence of propranolol (Prop) evoked positive inotropic response and ~3H-InsP accumulations, while stimulation of β-AR by isoprenaline (ISO) or NE in the presence of phentolamine (Phen) only evoked positive inotropic response, but not ~3H-InsP accumulations. (2) Simultaneous stimulation of α_1 and β-AR by NE or ISO plus PE significantly shifted the concentration-dependent inotropic response curves and ~3H-InsP accumulation curves to the left and upward compared with individual α_1-AR stimulation by PE or NE in the presence of Prop. (3) In the presence of ICI118551 (selective β_2-AR antagonist) or CGP12177 (selective β_1-AR antagonist), stimulation of either β_1 - or β_2-AR did not change α_1-AR-evoked inotropic response and ~3H-InsP accumulations. CONCLUSION: Stimulation of β_1-AR and β_2-AR potentiates α_1-AR-medi-ated positive inotropic response and InsP accumulation in isolated rat left atria.
机译：目的：研究刺激β-肾上腺素能受体（AR）及其亚型是否增强离体大鼠左心房中α_1-AR引起的正性肌力反应和肌醇磷酸酯（InsP）的积累。方法：通过收缩功能实验确定离体电驱动大鼠左心房的正性肌力反应。通过〜3H-肌醇掺入和柱色谱法测量〜3H-InsP积累。结果：（1）在存在心得安（Prop）的情况下，苯肾上腺素（PE）或去甲肾上腺素（NE）刺激α_1-AR引起正性肌力反应和〜3H-InsP积累，而异丙肾上腺素（ISO）刺激β-AR。苯妥拉明（Phen）时的NE或NE仅引起正性肌力反应，但不引起〜3H-InsP积累。 （2）与PE或NE单独刺激α_1-AR相比，NE或ISO加PE同时刺激α_1和β-AR显着地改变了浓度依赖性的肌力反应曲线和〜3H-InsP积累曲线。 （3）在存在ICI118551（选择性β_2-AR拮抗剂）或CGP12177（选择性β_1-AR拮抗剂）的情况下，刺激β_1-或β_2-AR不会改变α_1-AR引起的肌力反应和〜3H-InsP累积。结论：刺激β_1-AR和β_2-AR增强了α_1-AR介导的正性肌力反应和离体大鼠左心房的InsP蓄积。

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