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首页> 外文期刊>Acta Pharmacologica Sinica >Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats
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Upregulation of heparin-binding growth-associated molecule after spinal cord injury in adult rats

机译：成年大鼠脊髓损伤后肝素结合生长相关分子的上调

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摘要

AIM: To investigate whether traumatic spinal cord injury (SCI) induces changes of gene expression of heparin-binding growth-associated molecule (HB-GAM). METHODS: In a spinal cord transection model, HB-GAM expression and cellular localization were examined using Northern blot, RT-PCR, immunohistochemistry and immunofluorescence double-labeling methods. RESULTS: HB-GAM mRNA was significantly upregulated in spinal cord tissues rostral and caudal to the injury at 7 d after SCI. HB-GAM gene expression was markedly increased at 3 d, peaked at 7 d, and declined to the baseline level at 28 d post-injury. During its peak expression, HB-GAM was co-localized in astrocytes, oligodendrocytes, and neurons in spinal cord tissues within 7 mm from the site of spinal transection. CONCLUSION: SCI induces HB-GAM expression at both mRNA and protein levels in areas close to the injury. Both neurons and glial cells expressed HB-GAM implying that HB-GAM played a role in the process of injury and/or repair following SCI.
机译：目的：研究创伤性脊髓损伤（SCI）是否诱导肝素结合生长相关分子（HB-GAM）基因表达的变化。方法：在脊髓横断模型中，使用Northern印迹，RT-PCR，免疫组织化学和免疫荧光双重标记方法检测HB-GAM的表达和细胞定位。结果：脊髓损伤后7 d，大鼠脊髓尾状和尾状组织中的HB-GAM mRNA表达明显上调。 HB-GAM基因表达在伤后3 d明显增加，在7 d达到峰值，并在伤后28 d降至基线水平。在其高峰表达期间，HB-GAM共定位在距脊髓横断部位7 mm以内的脊髓组织中的星形胶质细胞，少突胶质细胞和神经元中。结论：脊髓损伤在邻近损伤区域的mRNA和蛋白水平均可诱导HB-GAM表达。神经元和神经胶质细胞均表达HB-GAM，这说明HB-GAM在SCI后的损伤和/或修复过程中发挥了作用。

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