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首页> 外文期刊>Acta Pharmacologica Sinica >Effect of Zn~(2+) ions on ryanodine binding to sarcoplasmic reticulum of striated muscles in the presence of pyrithione
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Effect of Zn~(2+) ions on ryanodine binding to sarcoplasmic reticulum of striated muscles in the presence of pyrithione

机译：巯基吡啶氧化物存在下Zn〜（2+）离子对ryanodine与横纹肌肌浆网结合的影响

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AIM: To explore whether the differential effects of Zn~(2+) on ryanodine binding to the sarcoplasmic reticulum (SR) of skeletal and cardiac muscles resulted from different permeability of the SR to Zn~(2+). METHODS: [~3H]ryanodine binding assays were performed to examine the effect of Zn~(2+) on ryanodine binding to the SR in the presence of pyrithione sodium (PyNa), a specific Zn~(2+) ionophore. RESULTS: As a control, PyNa up to 50 μmol/L did not induce any effect on ryanodine binding to the SR of cardiac muscle. But PyNa 1-100 μmol/L increased ryanodine binding in skeletal muscle with maximum binding (222.2%+-20.9 % of the control) and inhibited ryanodine binding to 50 % of the control at about 500 μmol/L. In the presence of PyNa 10 and 50 μmol/L the dose-dependence of the effect of Zn~(2+) in cardiac muscle was still monophasic and not changed by PyNa, while the biphasic effect of Zn~(2+) in skeletal muscle became monophasic. CONCLUSION: Different permeability of the SR to Zn~(2+) may account for the differential effects of Zn~(2+) on ryanodine binding in skeletal and cardiac muscles. PyNa is not a strictly specific Zn~(2+) ionophore.
机译：目的：探讨锌〜（2+）对雷诺定与骨骼肌和心肌肌浆网（SR）结合的差异作用是否是由于SR〜Zn〜（2+）的渗透性不同所致。方法：进行[〜3H] ryanodine结合试验，以检测在存在巯基吡啶氧化物钠（PyNa）（一种特定的Zn〜（2+）离子载体）的情况下Zn〜（2+）对ryanodine与SR结合的影响。结果：作为对照，高达50μmol/ L的PyNa不会对瑞丹定与心肌SR的结合产生任何影响。但是PyNa 1-100μmol/ L可以最大程度地增加骨骼肌中的ryanodine结合力（最大为对照组的222.2％+-20.9％），在约500μmol/ L时抑制ryanodine结合至对照组的50％。在存在PyNa 10和50μmol/ L的情况下，Zn〜（2+）在心肌中的剂量依赖性仍然是单相的，并且不受PyNa改变，而Zn〜（2+）在骨骼中的双相作用。肌肉变成单相的。结论：SR对Zn〜（2+）的渗透性不同可能是Zn〜（2+）对人体骨骼肌和心肌中ryanodine结合的不同影响。 PyNa不是严格特定的Zn〜（2+）离子载体。

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